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NLK elisa kit :: Human nemo-like kinase ELISA Kit

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Catalog # MBS9336671
Unit / Price
  48-Strip-Wells  /  $470 +1 FREE 8GB USB
  96-Strip-Wells  /  $680 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
NLK elisa kit
Product Name

nemo-like kinase (NLK), ELISA Kit

Also Known As

Human Serine/threonine-protein kinase NLK, NLK ELISA Kit

Product Synonym Names
Human Serine/threonine-protein kinase NLK (NLK) ELISA kit; DKFZp761G1211; FLJ21033; nemo like kinase; nemo-like kinase
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
609476
3D Structure
ModBase 3D Structure for Q9UBE8
Species Reactivity
Specificity
No significant cross-reactivity or interference between Human NLK and analogues was observed.
Samples
Serum, Plasma, Tissue Homogenate, Feces and Urine
Assay Type
Sandwich
Detection Range
0.25ng/ml-8ng/ml.
Sensitivity
0.1ng/ml.
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated NLK concentrations in Human serum, plasma and other body fluids. Using Purified Human NLK antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NLK and NLK antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NLK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of NLK elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forNLKpurchase
MBS9336671 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the nemo-like kinase (NLK) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NLK. The ELISA analytical biochemical technique of the MBS9336671 kit is based on NLK antibody-NLK antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NLK antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NLK. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
NLK elisa kit
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated NLK concentrations in Human serum, plasma and other body fluids. Using Purified Human NLK antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NLK and NLK antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NLK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS9336671. Click to request current manual
NCBI/Uniprot data below describe general gene information for NLK. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
58,283 Da
NCBI Official Full Name
serine/threonine-protein kinase NLK
NCBI Official Synonym Full Names
nemo-like kinase
NCBI Official Symbol
NCBI Protein Information
serine/threonine-protein kinase NLK
UniProt Protein Name
Serine/threonine-protein kinase NLK
UniProt Synonym Protein Names
Nemo-like kinase; Protein LAK1
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
NLK_HUMAN
UniProt Comments for NLK
NLK: Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK- SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors. Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Protein type: Kinase, protein; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.24; CMGC group; MAPK family; nmo subfamily

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: nucleoplasm; nucleus; cytosol

Molecular Function: MAP kinase activity; protein serine/threonine kinase activity; protein binding; ubiquitin protein ligase binding; magnesium ion binding; SH2 domain binding; transcription factor binding; ATP binding; protein kinase activity

Biological Process: Wnt receptor signaling pathway; negative regulation of Wnt receptor signaling pathway; regulation of transcription, DNA-dependent; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; protein amino acid autophosphorylation; peptidyl-threonine phosphorylation; MAPKKK cascade; protein amino acid phosphorylation; serine phosphorylation of STAT3 protein
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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