• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

NOS elisa kit :: Human nitric oxide synthase (NOS) ELISA Kit

Scan QR to view Datasheet
Catalog # MBS263712
Unit / Price
  48-Strip-Wells  /  $260 +1 FREE 8GB USB
  96-Strip-Wells  /  $405 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
Typical Testing Data/Standard Curve
Product Name

nitric oxide synthase (NOS), ELISA Kit

Popular Item
Also Known As

Human nitric oxide synthase (NOS) ELISA KIT

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
118
OMIM
163729
Species Reactivity
Specificity
No cross-reaction with other factors.
Samples
Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant NOS concentration.
Assay Type
Sandwich
Detection Range
20 ng/ml-0.312 ng/ml
Sensitivity
0.06 ng/ml.
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of NOS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forNOSpurchase
MBS263712 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the nitric oxide synthase (NOS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NOS. The ELISA analytical biochemical technique of the MBS263712 kit is based on NOS antibody-NOS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NOS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NOS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
NOS elisa kit
Principle of the Assay||This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human NOS monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Typical Testing Data/Standard Curve of NOS elisa kit
NOS elisa kit Typical Testing Data/Standard Curve image
Sample Manual Insert of MBS263712. Click to request current manual
NCBI/Uniprot data below describe general gene information for NOS. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
67,876 Da
NCBI Official Full Name
nitric oxide synthase, partial
NCBI Official Synonym Full Names
nitric oxide synthase 3 (endothelial cell)
NCBI Official Symbol
NCBI Official Synonym Symbols
eNOS; ECNOS
  [Similar Products]
NCBI Protein Information
nitric oxide synthase, endothelial; cNOS; EC-NOS; NOSIII; NOS type III; endothelial NOS; constitutive NOS; nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta21; nitric oxide synthase 3 transcript variant eNOS-delta20-21
UniProt Protein Name
Nitric oxide synthase, endothelial
UniProt Synonym Protein Names
Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
cNOS; eNOS; NOSIII  [Similar Products]
UniProt Entry Name
NOS3_HUMAN
NCBI Summary for NOS
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
UniProt Comments for NOS
eNOS: endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.

Protein type: EC 1.14.13.39; Amino Acid Metabolism - arginine and proline; Oxidoreductase

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: Golgi membrane; cytoskeleton; cytoplasm; plasma membrane; caveola; cytosol; nucleus

Molecular Function: actin monomer binding; calmodulin binding; protein binding; FAD binding; nitric-oxide synthase activity; FMN binding; NADPH-hemoprotein reductase activity; iron ion binding; heme binding; NADP binding; cadmium ion binding

Biological Process: regulation of systemic arterial blood pressure by endothelin; negative regulation of potassium ion transport; negative regulation of muscle hyperplasia; positive regulation of vasodilation; nitric oxide metabolic process; negative regulation of cell proliferation; regulation of the force of heart contraction by chemical signal; negative regulation of hydrolase activity; regulation of blood pressure; lipopolysaccharide-mediated signaling pathway; angiogenesis; negative regulation of blood pressure; nitric oxide biosynthetic process; regulation of blood vessel size; interaction with host; mitochondrion organization and biogenesis; negative regulation of calcium ion transport; in utero embryonic development; positive regulation of guanylate cyclase activity; regulation of sodium ion transport; ovulation from ovarian follicle; positive regulation of angiogenesis; response to heat; arginine catabolic process; endothelial cell migration; blood vessel remodeling; regulation of nitric-oxide synthase activity; blood coagulation; vascular endothelial growth factor receptor signaling pathway; nitric oxide mediated signal transduction; smooth muscle hyperplasia; lung development

Disease: Preeclampsia/eclampsia 1; Alzheimer Disease; Hypertension, Essential; Stroke, Ischemic
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000