• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

NOS1/nNOS elisa kit :: Mouse NOS1/nNOS ELISA Kit

Scan QR to view Datasheet
Catalog # MBS2513473
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

NOS1/nNOS, ELISA Kit

Popular Item
Also Known As

Mouse NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal) ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
1434
OMIM
163731
3D Structure
ModBase 3D Structure for P29475
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse NOS1/nNOS. No significant cross-reactivity or interference between Mouse NOS1/nNOS and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse NOS1/nNOS and all the analogues, therefore, cross reaction may still exist.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
Min: 0.094ng/mL; Max: 10ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of NOS1/nNOS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forNOS1/nNOSpurchase
MBS2513473 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the NOS1/nNOS, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NOS1/nNOS. The ELISA analytical biochemical technique of the MBS2513473 kit is based on NOS1/nNOS antibody-NOS1/nNOS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NOS1/nNOS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NOS1/nNOS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
NOS1/nNOS elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NOS1/nNOS in Mouse serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NOS1/nNOS. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse NOS1/nNOS and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NOS1/nNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse NOS1/nNOS. You can calculate the concentration of Mouse NOS1/nNOS in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of NOS1/nNOS elisa kit
NOS1/nNOS elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2513473. Click to request current manual
NCBI/Uniprot data below describe general gene information for NOS1/nNOS. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
164,779 Da
NCBI Official Full Name
nitric oxide synthase, brain isoform 1
NCBI Official Synonym Full Names
nitric oxide synthase 1 (neuronal)
NCBI Official Symbol
NCBI Official Synonym Symbols
NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
  [Similar Products]
NCBI Protein Information
nitric oxide synthase, brain; NOS type I; constitutive NOS; neuronal NOS; peptidyl-cysteine S-nitrosylase NOS1
UniProt Protein Name
Nitric oxide synthase, brain
UniProt Synonym Protein Names
Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; N-NOS; nNOS; Peptidyl-cysteine S-nitrosylase NOS1; bNOS
UniProt Gene Name
UniProt Synonym Gene Names
N-NOS; nNOS  [Similar Products]
UniProt Entry Name
NOS1_HUMAN
NCBI Summary for NOS1/nNOS
The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]
UniProt Comments for NOS1/nNOS
nNOS: nitric oxide synthase 1, neuronal form. Produces nitric oxide which is a messenger molecule with diverse functions and displays many properties of a neurotransmitter in the brain and peripheral nervous system. May be an effector enzyme for the dystrophin complex. Four alternatively spliced isoforms have been described.

Protein type: EC 1.14.13.39; Amino Acid Metabolism - arginine and proline; Oxidoreductase

Chromosomal Location of Human Ortholog: 12q24.22

Cellular Component: photoreceptor inner segment; cytoskeleton; protein complex; sarcoplasmic reticulum; mitochondrion; perinuclear region of cytoplasm; cytoplasm; dendritic spine; synapse; cytosol; sarcolemma; lipid raft

Molecular Function: calmodulin binding; protein binding; FAD binding; sodium channel regulator activity; FMN binding; NADPH-hemoprotein reductase activity; nitric-oxide synthase activity; iron ion binding; heme binding; oxidoreductase activity; NADP binding; cadmium ion binding

Biological Process: interaction with host; negative regulation of serotonin uptake; negative regulation of calcium ion transport; striated muscle contraction; positive regulation of transcription, DNA-dependent; negative regulation of potassium ion transport; positive regulation of guanylate cyclase activity; regulation of sodium ion transport; positive regulation of vasodilation; response to heat; negative regulation of hydrolase activity; exogenous drug catabolic process; response to hypoxia; peptidyl-cysteine S-nitrosylation; arginine catabolic process; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of blood pressure; blood coagulation; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; multicellular organismal response to stress; myoblast fusion; neurotransmitter biosynthetic process

Disease: Pyloric Stenosis, Infantile Hypertrophic, 1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000