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NOX 1 elisa kit :: Rat NOX1 ELISA Kit

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Catalog # MBS2508743
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  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
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Typical Testing Data/Standard Curve (for reference only)
Product Name

NOX1 (NOX 1), ELISA Kit

Popular Item
Full Product Name

Rat NOX1 (Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
300225
3D Structure
ModBase 3D Structure for Q9Y5S8
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat NOX 1. No significant cross-reactivity or interference between Rat NOX 1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rat NOX 1 and all the analogues, therefore, cross reaction may still exist.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.313-20ng/mL
Sensitivity
Min: 0.188ng/mL; Max: 20ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of NOX 1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for NOX 1purchase
MBS2508743 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the NOX1 (NOX 1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NOX 1. The ELISA analytical biochemical technique of the MBS2508743 kit is based on NOX 1 antibody-NOX 1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NOX 1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NOX 1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
NOX 1 elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NOX 1 in Rat serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NOX 1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat NOX 1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NOX 1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat NOX 1. You can calculate the concentration of Rat NOX 1 in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of NOX 1 elisa kit
NOX 1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2508743. Click to request current manual
NCBI/Uniprot data below describe general gene information for NOX 1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
58,972 Da
NCBI Official Full Name
NADPH oxidase 1 isoform 3
NCBI Official Synonym Full Names
NADPH oxidase 1
NCBI Official Symbol
NCBI Official Synonym Symbols
MOX1; NOH1; NOH-1; GP91-2
  [Similar Products]
NCBI Protein Information
NADPH oxidase 1; NADH/NADPH mitogenic oxidase subunit P65-MOX; NADPH oxidase homolog-1; mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating); mitogenic oxidase 1
UniProt Protein Name
NADPH oxidase 1
UniProt Synonym Protein Names
Mitogenic oxidase 1; MOX-1; NADH/NADPH mitogenic oxidase subunit P65-MOX; NOH-1
UniProt Gene Name
UniProt Synonym Gene Names
MOX1; NOH1; NOX-1; MOX-1  [Similar Products]
UniProt Entry Name
NOX1_HUMAN
NCBI Summary for NOX 1
This gene encodes a member of the NADPH oxidase family of enzymes responsible for the catalytic one-electron transfer of oxygen to generate superoxide or hydrogen peroxide. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012]
UniProt Comments for NOX 1
NOX1: NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; EC 1.-.-.-; Membrane protein, integral; Oxidoreductase; Channel, misc.; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: Xq22

Cellular Component: early endosome; integral to membrane; cell junction; NADPH oxidase complex

Molecular Function: protein binding; metal ion binding; superoxide-generating NADPH oxidase activity; Rac GTPase binding; NADP binding; voltage-gated proton channel activity

Biological Process: intracellular pH elevation; respiratory burst; cell migration; extracellular matrix organization and biogenesis; positive regulation of smooth muscle cell proliferation; superoxide metabolic process; positive regulation of JNK cascade; signal transduction; NADP metabolic process; regulation of systemic arterial blood pressure by renin-angiotensin; proton transport; regulation of blood pressure; positive regulation of cell proliferation; hydrogen peroxide metabolic process; positive regulation of integrin biosynthetic process; angiogenesis; inflammatory response; superoxide release
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with NOX 1 elisa kit
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