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NPC1 elisa kit :: Mouse Niemann-Pick disease, type C1 ELISA Kit

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Catalog # MBS906920
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Niemann-Pick disease, type C1 (NPC1), ELISA Kit

Popular Item
Also Known As

Mouse Niemann-Pick C1 protein, NPC1 ELISA Kit

Product Synonym Names
Mouse Niemann-Pick C1 protein (NPC1) ELISA kit; FLJ98532; NPC; ; Niemann-Pick disease; type C1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS906920 Testing Data
Sequence Length
1277
3D Structure
ModBase 3D Structure for O35604
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse NPC1. No significant cross-reactivity or interference between Mouse NPC1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
31.25 pg/ml-2000 pg/ml
Sensitivity
7.81 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of NPC1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forNPC1purchase
MBS906920 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Niemann-Pick disease, type C1 (NPC1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NPC1. The ELISA analytical biochemical technique of the MBS906920 kit is based on NPC1 antibody-NPC1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NPC1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NPC1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
NPC1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NPC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NPC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NPC1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NPC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of NPC1 elisa kit
NPC1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS906920. Click to request current manual
NCBI/Uniprot data below describe general gene information for NPC1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
142,885 Da
NCBI Official Full Name
Niemann-Pick C1 protein
NCBI Official Synonym Full Names
Niemann-Pick type C1
NCBI Official Symbol
NCBI Official Synonym Symbols
spm; lcsd; C85354; nmf164; D18Ertd139e; D18Ertd723e; A430089E03Rik
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NCBI Protein Information
Niemann-Pick C1 protein; sphingomyelinosis; Niemann Pick type C1
UniProt Protein Name
Niemann-Pick C1 protein
Protein Family
UniProt Gene Name
UniProt Entry Name
NPC1_MOUSE
UniProt Comments for NPC1
NPC1: Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes/ lysosomes to the ER and plasma membrane by an unknown mechanism. Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals. Defects in NPC1 are the cause of Niemann-Pick disease type C1 (NPC1). A lysosomal storage disorder that affects the viscera and the central nervous system. It is due to defective intracellular processing and transport of low-density lipoprotein derived cholesterol. It causes accumulation of cholesterol in lysosomes, with delayed induction of cholesterol homeostatic reactions. Niemann-Pick disease type C1 has a highly variable clinical phenotype. Clinical features include variable hepatosplenomegaly and severe progressive neurological dysfunction such as ataxia, dystonia and dementia. The age of onset can vary from infancy to late adulthood. An allelic variant of Niemann-Pick disease type C1 is found in people with Nova Scotia ancestry. Patients with the Nova Scotian clinical variant are less severely affected. Belongs to the patched family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Cellular Component: Golgi apparatus; lysosomal membrane; endoplasmic reticulum; lysosome; integral to plasma membrane; integral to membrane; extracellular region; nuclear envelope; lipid raft; membrane; perinuclear region of cytoplasm; plasma membrane; endosome; vesicle

Molecular Function: protein binding; hedgehog receptor activity; cholesterol binding

Biological Process: response to drug; steroid metabolic process; cholesterol metabolic process; cholesterol transport; protein amino acid glycosylation; endocytosis; cholesterol efflux; adult walking behavior; negative regulation of macroautophagy; cholesterol homeostasis; response to cadmium ion; autophagy; lipid metabolic process; lipid raft organization and biogenesis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with NPC1 elisa kit
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