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NPC2 elisa kit :: Mouse Epididymal secretory protein E1 (NPC2) ELISA Kit

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Catalog # MBS7206139
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Epididymal secretory protein E1 (NPC2), ELISA Kit

Popular Item
Also Known As

Mouse Epididymal secretory protein E1 (NPC2) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 607625
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of NPC2. No significant cross-reactivity or interference between NPC2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NPC2 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Competitive (Quantitative)
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of NPC2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forNPC2purchase
MBS7206139 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Epididymal secretory protein E1 (NPC2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NPC2. The ELISA analytical biochemical technique of the MBS7206139 kit is based on NPC2 antibody-NPC2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NPC2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NPC2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
NPC2 elisa kit
Intended Uses: This NPC2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse NPC2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: NPC2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-NPC2 antibody and an NPC2-HRP conjugate. The assay sample and buffer are incubated together with NPC2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NPC2 concentration since NPC2 from samples and NPC2-HRP conjugate compete for the anti-NPC2 antibody binding site. Since the number of sites is limited, as more sites are occupied by NPC2 from the sample, fewer sites are left to bind NPC2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NPC2 concentration in each sample is interpolated from this standard curve.

Typical Testing Data/Standard Curve (for reference only) of NPC2 elisa kit
NPC2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS7206139. Click to request current manual
NCBI/Uniprot data below describe general gene information for NPC2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
16,570 Da
NCBI Official Full Name
epididymal secretory protein E1
NCBI Official Synonym Full Names
Niemann-Pick disease, type C2
NCBI Official Symbol
NCBI Official Synonym Symbols
HE1; EDDM1
  [Similar Products]
NCBI Protein Information
epididymal secretory protein E1; epididymal protein 1; tissue-specific secretory protein; human epididymis-specific protein 1; Niemann-Pick disease type C2 protein
UniProt Protein Name
Epididymal secretory protein E1
UniProt Synonym Protein Names
Human epididymis-specific protein 1; He1; Niemann-Pick disease type C2 protein
UniProt Gene Name
UniProt Synonym Gene Names
HE1; He1  [Similar Products]
UniProt Entry Name
NPC2_HUMAN
NCBI Summary for NPC2
This gene encodes a protein containing a lipid recognition domain. The encoded protein may function in regulating the transport of cholesterol through the late endosomal/lysosomal system. Mutations in this gene have been associated with Niemann-Pick disease, type C2 and frontal lobe atrophy. [provided by RefSeq, Jul 2008]
UniProt Comments for NPC2
NPC2: Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes/ lysosomes to the ER and plasma membrane by an unknown mechanism. The secreted form of NCP2 regulates biliary cholesterol secretion via stimulation of ABCG5/ABCG8-mediated cholesterol transport. Defects in NPC2 are the cause of Niemann-Pick disease type C2 (NPDC2). A lysosomal storage disorder that affects the viscera and the central nervous system. It is due to defective intracellular processing and transport of low-density lipoprotein derived cholesterol. It causes accumulation of cholesterol in lysosomes, with delayed induction of cholesterol homeostatic reactions. Niemann-Pick disease type C2 has a highly variable clinical phenotype. Clinical features include variable hepatosplenomegaly and severe progressive neurological dysfunction such as ataxia, dystonia and dementia. The age of onset can vary from infancy to late adulthood. Belongs to the NPC2 family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: lysosome; endoplasmic reticulum

Molecular Function: protein binding; enzyme binding; cholesterol binding

Biological Process: cholesterol metabolic process; cholesterol homeostasis; cholesterol transport; intracellular sterol transport; regulation of isoprenoid metabolic process; response to virus; intracellular cholesterol transport; cholesterol efflux; phospholipid transport; glycolipid transport

Disease: Niemann-pick Disease, Type C2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with NPC2 elisa kit
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