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NR1H2 elisa kit :: Human nuclear receptor subfamily 1, group H, member 2 ELISA Kit

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Catalog # MBS701505
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Typical Testing Data/Standard Curve (for reference only)
Product Name

nuclear receptor subfamily 1, group H, member 2 (NR1H2), ELISA Kit

Popular Item
Also Known As

Human Liver X Receptor beta, LXRbeta ELISA Kit

Product Synonym Names
Human Liver X Receptor beta (LXRbeta) ELISA Kit; LXR-b; LXRB; NER; NER-I; RIP15; UNR; LX receptor beta; liver X receptor beta; liver X receptor-beta; nuclear orphan receptor LXR-beta; oxysterols receptor LXR-beta; steroid hormone-nuclear receptor NER; u; nuclear receptor subfamily 1; group H; member 2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS701505 Testing Data
Sequence Length
364
OMIM
600380
3D Structure
ModBase 3D Structure for P55055
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human NR1H2. No significant cross-reactivity or interference between Human NR1H2 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
31.25 pg/ml-2000 pg/ml
Sensitivity
7.81 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Transcription/Transcription regulation
Protein Biological Process 3
Transcription
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of NR1H2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forNR1H2purchase
MBS701505 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the nuclear receptor subfamily 1, group H, member 2 (NR1H2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NR1H2. The ELISA analytical biochemical technique of the MBS701505 kit is based on NR1H2 antibody-NR1H2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NR1H2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NR1H2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
NR1H2 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LXRbeta has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LXRbeta present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LXRbeta is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LXRbeta bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of NR1H2 elisa kit
NR1H2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS701505. Click to request current manual
NCBI/Uniprot data below describe general gene information for NR1H2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
50,974 Da
NCBI Official Full Name
oxysterols receptor LXR-beta isoform 2
NCBI Official Synonym Full Names
nuclear receptor subfamily 1, group H, member 2
NCBI Official Symbol
NR1H2  [Similar Products]
NCBI Official Synonym Symbols
NER; UNR; LXRB; LXR-b; NER-I; RIP15
  [Similar Products]
NCBI Protein Information
oxysterols receptor LXR-beta; LX receptor beta; nuclear receptor NER; liver X nuclear receptor beta; nuclear orphan receptor LXR-beta; steroid hormone-nuclear receptor NER; ubiquitously-expressed nuclear receptor
UniProt Protein Name
Oxysterols receptor LXR-beta
UniProt Synonym Protein Names
Liver X receptor beta; Nuclear receptor NER; Nuclear receptor subfamily 1 group H member 2; Ubiquitously-expressed nuclear receptor
Protein Family
UniProt Gene Name
NR1H2  [Similar Products]
UniProt Synonym Gene Names
LXRB; NER; UNR  [Similar Products]
UniProt Entry Name
NR1H2_HUMAN
NCBI Summary for NR1H2
The liver X receptors, LXRA (NR1H3; MIM 602423) and LXRB, form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. The inducible LXRA is highly expressed in liver, adrenal gland, intestine, adipose tissue, macrophages, lung, and kidney, whereas LXRB is ubiquitously expressed. Ligand-activated LXRs form obligate heterodimers with retinoid X receptors (RXRs; see MIM 180245) and regulate expression of target genes containing LXR response elements (summary by Korf et al., 2009 [PubMed 19436111]).[supplied by OMIM, Jan 2010]
UniProt Comments for NR1H2
Function: Nuclear receptor. Binds preferentially to double-stranded oligonucleotide direct repeats having the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). Regulates cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8; DLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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