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NR4A2 elisa kit :: Human nuclear receptor subfamily 4, group A, member 2 ELISA Kit

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Catalog # MBS912795
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

nuclear receptor subfamily 4, group A, member 2 (NR4A2), ELISA Kit

Popular Item
Also Known As

Human Nuclear receptor subfamily 4 group A member 2, NR4A2 ELISA Kit

Product Synonym Names
Human Nuclear receptor subfamily 4 group A member 2 (NR4A2) ELISA kit; HZF-3; NOT; NURR1; RNR1; TINUR; NGFI-B/nur77 beta-type transcription factor homolog; OTTHUMP00000204707; T-cell nuclear receptor NOT; intermediate-early receptor protein; nur related protein-1; human ho; nuclear receptor subfamily 4; group A; member 2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS912795 Testing Data
Sequence Length
598
OMIM
601828
3D Structure
ModBase 3D Structure for P43354
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human NR4A2. No significant cross-reactivity or interference between human NR4A2 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, cell lysates.
Assay Type
Sandwich
Detection Range
31.25 pg/ml -2000 pg/ml.
Sensitivity
The minimum detectable dose of human NR4A2 is typically less than 7.81 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of NR4A2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forNR4A2purchase
MBS912795 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the nuclear receptor subfamily 4, group A, member 2 (NR4A2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NR4A2. The ELISA analytical biochemical technique of the MBS912795 kit is based on NR4A2 antibody-NR4A2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NR4A2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NR4A2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
NR4A2 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NR4A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NR4A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NR4A2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NR4A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of NR4A2 elisa kit
NR4A2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS912795. Click to request current manual
NCBI/Uniprot data below describe general gene information for NR4A2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
66,591 Da
NCBI Official Full Name
nuclear receptor subfamily 4 group A member 2
NCBI Official Synonym Full Names
nuclear receptor subfamily 4, group A, member 2
NCBI Official Symbol
NR4A2  [Similar Products]
NCBI Official Synonym Symbols
NOT; RNR1; HZF-3; NURR1; TINUR
  [Similar Products]
NCBI Protein Information
nuclear receptor subfamily 4 group A member 2; nuclear receptor related 1; T-cell nuclear receptor NOT; orphan nuclear receptor NR4A2; orphan nuclear receptor NURR1; intermediate-early receptor protein; immediate-early response protein NOT; nur related protein-1, human homolog of; transcriptionally-inducible nuclear receptor; NGFI-B/nur77 beta-type transcription factor homolog; transcriptionally inducible nuclear receptor related 1
UniProt Protein Name
Nuclear receptor subfamily 4 group A member 2
UniProt Synonym Protein Names
Immediate-early response protein NOT; Orphan nuclear receptor NURR1; Transcriptionally-inducible nuclear receptor
UniProt Gene Name
NR4A2  [Similar Products]
UniProt Synonym Gene Names
NOT; NURR1; TINUR  [Similar Products]
UniProt Entry Name
NR4A2_HUMAN
NCBI Summary for NR4A2
This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. The encoded protein may act as a transcription factor. Mutations in this gene have been associated with disorders related to dopaminergic dysfunction, including Parkinson disease, schizophernia, and manic depression. Misregulation of this gene may be associated with rheumatoid arthritis. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
UniProt Comments for NR4A2
NR4A2: Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. Belongs to the nuclear hormone receptor family. NR4 subfamily.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 2q22-q23

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; ligand-dependent nuclear receptor activity; zinc ion binding; protein heterodimerization activity; steroid hormone receptor activity

Biological Process: positive regulation of catalytic activity; transcription initiation from RNA polymerase II promoter; response to inorganic substance; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; adult locomotory behavior; death; regulation of dopamine metabolic process; response to amphetamine; neuron migration; central nervous system projection neuron axonogenesis; dopamine biosynthetic process; response to insecticide; signal transduction; neuron maturation; post-embryonic development; habenula development; regulation of respiratory gaseous exchange; general adaptation syndrome; cellular response to extracellular stimulus; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; gene expression; negative regulation of neuron apoptosis

Disease: Parkinson Disease, Late-onset
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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