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NRF1 elisa kit :: Mouse nuclear respiratory factor 1 ELISA Kit

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Catalog # MBS915302
Unit / Price DISCONTINUED
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Typical Testing Data/Standard Curve (for reference only)
Product Name

nuclear respiratory factor 1 (NRF1), ELISA Kit

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Also Known As

Mouse Nuclear respiratory factor 1, NRF1 ELISA Kit

Product Synonym Names
Mouse Nuclear respiratory factor 1 (NRF1) ELISA kit; ALPHA-PAL; OTTHUMP00000184912; alpha palindromic-binding protein; nuclear respiratory factor 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS915302 Testing Data
Sequence Length
503
3D Structure
ModBase 3D Structure for Q9WU00
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse NRF1. No significant cross-reactivity or interference between mouse NRF1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
15.6 pg/ml -1000 pg/ml.
Sensitivity
The minimum detectable dose of mouse NRF1 is typically less than 3.9 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of NRF1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forNRF1purchase
MBS915302 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the nuclear respiratory factor 1 (NRF1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NRF1. The ELISA analytical biochemical technique of the MBS915302 kit is based on NRF1 antibody-NRF1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NRF1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NRF1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
NRF1 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NRF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NRF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NRF1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NRF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of NRF1 elisa kit
NRF1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS915302. Click to request current manual
NCBI/Uniprot data below describe general gene information for NRF1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
53,571 Da
NCBI Official Full Name
nuclear respiratory factor 1 isoform a
NCBI Official Synonym Full Names
nuclear respiratory factor 1
NCBI Official Symbol
NCBI Official Synonym Symbols
C87038; D6Ertd415e
  [Similar Products]
NCBI Protein Information
nuclear respiratory factor 1; NRF-1; alpha-pal; alpha palindromic-binding protein
UniProt Protein Name
Nuclear respiratory factor 1
UniProt Synonym Protein Names
Alpha palindromic-binding protein; Alpha-pal
UniProt Gene Name
UniProt Synonym Gene Names
NRF-1; Alpha-pal  [Similar Products]
UniProt Entry Name
NRF1_MOUSE
UniProt Comments for NRF1
NRF1: Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. Belongs to the NRF1/Ewg family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Cellular Component: transcription factor complex; cytoplasm; nucleus

Molecular Function: DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; mitochondrion organization and biogenesis; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; response to electrical stimulus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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