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NRIP1 elisa kit :: Mouse Nuclear Receptor Interacting Protein 1 ELISA Kit

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Catalog # MBS727673
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Nuclear Receptor Interacting Protein 1 (NRIP1), ELISA Kit

Popular Item
Also Known As

Mouse Nuclear Receptor Interacting Protein 1 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
602490
Species Reactivity
Samples
Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of NRIP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forNRIP1purchase
MBS727673 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Nuclear Receptor Interacting Protein 1 (NRIP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NRIP1. The ELISA analytical biochemical technique of the MBS727673 kit is based on NRIP1 antibody-NRIP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NRIP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NRIP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
NRIP1 elisa kit
For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma

Intended Uses: This NRIP1 ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of NRIP1 in the sample, this NRIP1 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus NRIP1 concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of NRIP1 utilizes a multiclonal anti-NRIP1 antibody and an NRIP1-HRP conjugate. The assay sample and buffer are incubated together with NRIP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NRIP1 concentration since NRIP1 from samples and NRIP1-HRP conjugate compete for the anti-NRIP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by NRIP1 from the sample, fewer sites are left to bind NRIP1-HRP conjugate. Standards of known NRIP1 concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of NRIP1. The NRIP1 concentration in each sample is interpolated from this standard curve.

Typical Testing Data/Standard Curve (for reference only) of NRIP1 elisa kit
NRIP1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS727673. Click to request current manual
NCBI/Uniprot data below describe general gene information for NRIP1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
126,942 Da
NCBI Official Full Name
nuclear receptor-interacting protein 1
NCBI Official Synonym Full Names
nuclear receptor interacting protein 1
NCBI Official Symbol
NRIP1  [Similar Products]
NCBI Official Synonym Symbols
RIP140
  [Similar Products]
NCBI Protein Information
nuclear receptor-interacting protein 1; nuclear factor RIP140; receptor interacting protein 140; receptor-interacting protein 140
UniProt Protein Name
Nuclear receptor-interacting protein 1
UniProt Synonym Protein Names
Nuclear factor RIP140; Receptor-interacting protein 140
UniProt Gene Name
NRIP1  [Similar Products]
UniProt Entry Name
NRIP1_HUMAN
NCBI Summary for NRIP1
Nuclear receptor interacting protein 1 (NRIP1) is a nuclear protein that specifically interacts with the hormone-dependent activation domain AF2 of nuclear receptors. Also known as RIP140, this protein modulates transcriptional activity of the estrogen receptor. [provided by RefSeq, Jul 2008]
UniProt Comments for NRIP1
RIP140: Modulates transcriptional activation by steroid receptors such as NR3C1, NR3C2 and ESR1. Also modulates transcriptional repression by nuclear hormone receptors. Interacts with RARA and RXRB homodimers and RARA/RXRB heterodimers in the presence of ligand. Interacts with HDAC1 and HDAC3 via its N-terminal domain. Interacts with NR2C1 (sumoylated form and via the ligand-binding domain); the interaction results in promoting the repressor activity of NR2C1. Interacts with CTBP1, CTBP2, ESR1, HDAC1, HDAC2, HDAC5, HDAC6, NR2C2, NR3C1, NR3C2, YWHAH, JUN and FOS. Found in a complex with both NR3C1 and YWHAH. Interacts with ZNF366.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 21q11.2

Cellular Component: nucleoplasm; histone deacetylase complex; nucleolus; nuclear speck; cell junction; nucleus

Molecular Function: retinoid X receptor binding; protein binding; androgen receptor binding; histone deacetylase binding; transcription coactivator activity; estrogen receptor binding; transcription corepressor activity; nuclear hormone receptor binding; glucocorticoid receptor binding

Biological Process: circadian rhythm; sequestering of lipid; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; negative regulation of transcription from RNA polymerase II promoter; ovarian follicle rupture
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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