• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

NSMCE2 elisa kit :: Human non-SMC element 2, MMS21 homolog (S. cerevisiae) ELISA Kit

Scan QR to view Datasheet
Catalog # MBS917697
Unit / Price DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Typical Testing Data/Standard Curve (for reference only)
Product Name

non-SMC element 2, MMS21 homolog (S. cerevisiae) (NSMCE2), ELISA Kit

Popular Item
Also Known As

Human E3 SUMO-protein ligase NSE2, NSMCE2 ELISA Kit

Product Synonym Names
Human E3 SUMO-protein ligase NSE2 (NSMCE2) ELISA kit; C8orf36; FLJ32440; MMS21; NSE2; methyl methanesulfonate sensitivity gene 21; non-SMC element 2; MMS21 homolog; non-SMC element 2; MMS21 homolog (S. cerevisiae)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS917697 Testing Data
Sequence Length
247
3D Structure
ModBase 3D Structure for Q96MF7
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human NSMCE2. No significant cross-reactivity or interference between human NSMCE2 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
28 pg/ml-1800 pg/ml.
Sensitivity
The minimum detectable dose of human NSMCE2 is typically less than 7 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of NSMCE2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forNSMCE2purchase
MBS917697 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the non-SMC element 2, MMS21 homolog (S. cerevisiae) (NSMCE2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NSMCE2. The ELISA analytical biochemical technique of the MBS917697 kit is based on NSMCE2 antibody-NSMCE2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NSMCE2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NSMCE2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
NSMCE2 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NSMCE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NSMCE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NSMCE2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NSMCE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of NSMCE2 elisa kit
NSMCE2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS917697. Click to request current manual
NCBI/Uniprot data below describe general gene information for NSMCE2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
27,932 Da
NCBI Official Full Name
E3 SUMO-protein ligase NSE2
NCBI Official Synonym Full Names
non-SMC element 2, MMS21 homolog (S. cerevisiae)
NCBI Official Symbol
NSMCE2  [Similar Products]
NCBI Official Synonym Symbols
NSE2; MMS21; ZMIZ7; C8orf36
  [Similar Products]
NCBI Protein Information
E3 SUMO-protein ligase NSE2; hMMS21; MMS21 homolog; non-SMC element 2 homolog; zinc finger, MIZ-type containing 7; methyl methanesulfonate sensitivity gene 21; non-structural maintenance of chromosomes element 2 homolog
UniProt Protein Name
E3 SUMO-protein ligase NSE2
UniProt Synonym Protein Names
MMS21 homolog; hMMS21; Non-structural maintenance of chromosomes element 2 homolog; Non-SMC element 2 homolog
Protein Family
UniProt Gene Name
NSMCE2  [Similar Products]
UniProt Synonym Gene Names
C8orf36; MMS21; hMMS21; Non-SMC element 2 homolog  [Similar Products]
UniProt Entry Name
NSE2_HUMAN
UniProt Comments for NSMCE2
NSMCE2: E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand break repair by homologous recombination. Is not be required for the stability of the complex. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, the shelterin complex subunits TERF1, TERF2, TINF2 and TERF2IP, and maybe the cohesin components RAD21 and STAG2. Required for recruitment of telomeres to PML nuclear bodies. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair, and for formation of APBs in ALT cell lines. Required for sister chromatid cohesion during prometaphase and mitotic progression. Belongs to the NSE2 family.

Protein type: Ubiquitin ligase; SUMO conjugating system; EC 6.3.2.-; EC 6.-.-.-; Ligase

Chromosomal Location of Human Ortholog: 8q24.13

Cellular Component: chromosome, telomeric region; PML body; nucleus

Molecular Function: protein binding; zinc ion binding; SUMO ligase activity; ligase activity

Biological Process: mitosis; protein sumoylation; positive regulation of maintenance of mitotic sister chromatid cohesion; cell division; telomere maintenance via recombination; positive regulation of mitotic metaphase/anaphase transition; double-strand break repair via nonhomologous end joining; double-strand break repair via homologous recombination
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Diseases associated with NSMCE2 elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1 publications with NSMCE2 and Neoplasms
Organs/Tissues associated with NSMCE2 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >1 publications with NSMCE2 and Brain
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000