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OC recombinant protein :: Osteocalcin (OC) Recombinant Protein

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Catalog # MBS2009075 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
Scan QR to view Datasheet
  0.01 mg  /  $140 +1 FREE 8GB USB
  0.05 mg  /  $265 +1 FREE 8GB USB
  0.1 mg  /  $410 +1 FREE 8GB USB
  0.2 mg  /  $505 +1 FREE 8GB USB
  0.5 mg  /  $980 +1 FREE 8GB USB
  1 mg  /  $1,445 +1 FREE 8GB USB
Product Name

Osteocalcin (OC), Recombinant Protein

Popular Item
Full Product Name

Recombinant Osteocalcin (OC)

Matching Pairs
Matching Pairs
APC-CY7 Conjugated Antibody: Osteocalcin (OC) (MBS2042743)
Immunogen: Osteocalcin (OC) (MBS2009075)
Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF - ALCLSDL TDAKPSGPES DKAFMSKQEG NKVVNRLRRY LGASVPSPDP LEPTREQCEL NPACDELSDQ YGLKTAYKRI YGITI
Chromosome Location
Chromosome: 3; NC_000069.6 (88383495..88384466, complement). Location: 3 F1; 3 38.82 cM
3D Structure
ModBase 3D Structure for P86546
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as freeze-dried powder form in Phosphate buffered saline(PBS), pH 7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
Organism
Mus musculus (Mouse)
Expression System
Prokaryotic expression
Residues
Ala14~Ile95 (Accession # P86546) with two N-terminal Tags, His-tag and GST-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of OC recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
OC recombinant protein
About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Applications Tested/Suitable for OC recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

Sequence Information of OC recombinant protein
OC recombinant protein Sequence Information image
NCBI/Uniprot data below describe general gene information for OC. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
41.0kDa
NCBI Official Full Name
osteocalcin isoform 1
NCBI Official Synonym Full Names
bone gamma carboxyglutamate protein
NCBI Official Symbol
Bglap  [Similar Products]
NCBI Official Synonym Symbols
OC; OG1; mOC-A; Bglap1
  [Similar Products]
NCBI Protein Information
osteocalcin; BGP; bone Gla protein; bone gamma carboxyglutamate protein 1; gamma-carboxyglutamic acid-containing protein
UniProt Protein Name
Osteocalcin
UniProt Synonym Protein Names
Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein
UniProt Gene Name
Bglap  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
OSTCN_MOUSE
UniProt Comments for OC
Function: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.

Subcellular location: Secreted.

Tissue specificity: Bone.

Post-translational modification: Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.

Sequence similarities: Belongs to the osteocalcin/matrix Gla protein family.Contains 1 Gla (gamma-carboxy-glutamate) domain.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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