View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List ODN ligand Catalog # MBS668911 Unit / Price 0.1 mg / $235 +1 FREE 8GB USB 5x0.1 mg / $910 +1 FREE 8GB USB Previous Next Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name ODN, Ligand Popular Item Full Product Name CpG ODN (1826), TLR9 ligand (Class B) Product Gene Name ODN ligand[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Species Reactivity Rat, Mouse Form/Format 1 mg/ml in entotoxin-free water Preparation and Storage Upon receipt, store at -20 degree C (Stable for at least 6 months).Avoid frequent freeze/thaw cycles. Other Notes Small volumes of ODN ligand vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for ODN ligand Sequence: 5'-tccatgaCGttcctgaCGtt-3' (Class B ODN 1826: Bases are phosphorothioate.) Synthetic oligodeoxynucleotides (ODN) containing unmethylated deoxycytosine-deoxyguanosine (CpG) motif are equivalent to bacterial DNA in the immunostimulatory activity, which can induce innate immunity via Toll-like receptor 9 (TLR9) in mammals. There are three major classes of CpG ODNs, and each ODN class exhibits different stimulatory effects on immune cell activation. Class A ODNs are potent inducers of IFN-alpha that leads to the plasmacytoid dendritc cell (pDC) maturation. Class B ODNs are relatively weak inducers of type I IFNs but strong stimulators of human B cells and monocyte maturation. Class C ODNs combine elements of both Classes A and B ODNs, which can induce IFN-alpha in pDC and activation of B cells. Product Categories/Family for ODN ligand Ligands and Inhibitors; TLR Ligands Applications Tested/Suitable for ODN ligand Functional Assay Application Notes for ODN ligand B. CpG ODN 1826-mediated mouse TLR9 activation in NF-kBLeeporter; RAW 264.7 cells (Figure 2). 1. Harvest NF-kB Leeporter; RAW 264.7 cells and seed cells into a white solid-bottom 96-well microplate in 100 ul of growth medium at 5 x 104cells/well. 2. Incubate cells at 37 degree C in a CO2incubator for overnight. 3. The next day, stimulate cells with various amounts of CpG ODN 1826. 4. Incubate at 37 degree C in a CO2incubator for 6-16 hours. 5. Add 30-50 ul of luciferase assay reagent per well. 6. Incubate at room temperature for 1-5 minutes and measure luminescence using a microplate luminometer. Structure of ODN ligand Fig-1: chemical structure of CpG ODN Testing Data of ODN ligand Fig-2. Induction of mouse TLR9 activity by CpG ODN 1826. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP