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OPG elisa kit :: Human Osteoprotegerin ELISA Kit

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Catalog # MBS763642
Unit / Price
  48-Strip-Wells  /  $300 +1 FREE 8GB USB
  96-Strip-Wells  /  $415 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
Product Name

Osteoprotegerin (OPG), ELISA Kit

Popular Item
Also Known As

Human Osteoprotegerin ELISA Kit

Product Synonym Names
OPG (Osteoprotegerin)/OCIF/TNFRSF11B/OPGtumor necrosis factor receptor superfamily member 11B/Osteoclastogenesis inhibitory factor/Osteoprotegerin/TR1/tumor necrosis factor receptor superfamily; member 11b
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
401
OMIM
239000
Species Reactivity
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
62.5-4000pg/ml
Sensitivity
37.5pg/ml
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of OPG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forOPGpurchase
MBS763642 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Osteoprotegerin (OPG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing OPG. The ELISA analytical biochemical technique of the MBS763642 kit is based on OPG antibody-OPG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OPG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OPG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
OPG elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-human OPGantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-human OPG antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human OPG amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofhuman OPGcan be calculated.

Typical Testing Data of OPG elisa kit
OPG elisa kit Typical Testing Data image
Typical Standard Curve of OPG elisa kit
OPG elisa kit Typical Standard Curve image
Sample Manual Insert of MBS763642. Click to request current manual
NCBI/Uniprot data below describe general gene information for OPG. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
46,026 Da
NCBI Official Full Name
osteoprotegerin
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily member 11b
NCBI Official Symbol
TNFRSF11B  [Similar Products]
NCBI Official Synonym Symbols
OPG; TR1; OCIF; PDB5
  [Similar Products]
NCBI Protein Information
tumor necrosis factor receptor superfamily member 11B
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 11B
UniProt Synonym Protein Names
Osteoclastogenesis inhibitory factor; Osteoprotegerin
UniProt Gene Name
TNFRSF11B  [Similar Products]
UniProt Synonym Gene Names
OCIF; OPG  [Similar Products]
UniProt Entry Name
TR11B_HUMAN
NCBI Summary for OPG
The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. [provided by RefSeq, Jul 2008]
UniProt Comments for OPG
TNFRSF11B: Acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis. Defects in TNFRSF11B are the cause of juvenile Paget disease (JPD); also known as hyperostosis corticalis deformans juvenilis or hereditary hyperphosphatasia or chronic congenital idiopathic hyperphosphatasia. JPD is a rare autosomal recessive osteopathy that presents in infancy or early childhood. The disorder is characterized by rapidly remodeling woven bone, osteopenia, debilitating fractures, and deformities due to a markedly accelerated rate of bone remodeling throughout the skeleton. Approximately 40 cases of JPD have been reported worldwide. Unless it is treated with drugs that block osteoclast- mediated skeletal resorption, the disease can be fatal.

Protein type: Secreted, signal peptide; Inhibitor; Secreted

Chromosomal Location of Human Ortholog: 8q24

Cellular Component: extracellular region; extracellular space; integral to plasma membrane; plasma membrane; proteinaceous extracellular matrix

Molecular Function: cytokine activity; receptor activity; tumor necrosis factor receptor activity

Biological Process: extracellular matrix organization and biogenesis; immune response; inflammatory response; negative regulation of bone resorption; negative regulation of odontogenesis of dentine-containing teeth; positive regulation of MAPKKK cascade; regulation of apoptosis; regulation of cell proliferation; response to arsenic; response to drug; response to estrogen stimulus; response to lipopolysaccharide; response to magnesium ion; response to nutrient; signal transduction; skeletal development; tumor necrosis factor-mediated signaling pathway

Disease: Paget Disease, Juvenile
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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