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OPRD1 elisa kit :: Mouse Delta-type opioid receptor (OPRD1) ELISA Kit

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Catalog # MBS7234849
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Delta-type opioid receptor (OPRD1), ELISA Kit

Popular Item
Also Known As

Mouse Delta-type opioid receptor (OPRD1) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
165195
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of OPRD1. No significant cross-reactivity or interference between OPRD1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between OPRD1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of OPRD1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forOPRD1purchase
MBS7234849 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Delta-type opioid receptor (OPRD1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing OPRD1. The ELISA analytical biochemical technique of the MBS7234849 kit is based on OPRD1 antibody-OPRD1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OPRD1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OPRD1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
OPRD1 elisa kit
Intended Uses: This OPRD1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse OPRD1.

Principle of the Assay: OPRD1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-OPRD1 antibody and an OPRD1-HRP conjugate. The assay sample and buffer are incubated together with OPRD1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OPRD1 concentration since OPRD1 from samples and OPRD1-HRP conjugate compete for the anti-OPRD1 antibody binding site. Since the number of sites is limited, as more sites are occupied by OPRD1 from the sample, fewer sites are left to bind OPRD1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OPRD1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for OPRD1 elisa kit

Typical Testing Data/Standard Curve (for reference only) of OPRD1 elisa kit
OPRD1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS7234849. Click to request current manual
NCBI/Uniprot data below describe general gene information for OPRD1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
40,369 Da
NCBI Official Full Name
delta-type opioid receptor
NCBI Official Synonym Full Names
opioid receptor, delta 1
NCBI Official Symbol
OPRD1  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
delta-type opioid receptor; DOR-1; D-OR-1; delta opioid receptor 1
UniProt Protein Name
Delta-type opioid receptor
UniProt Gene Name
OPRD1  [Similar Products]
UniProt Synonym Gene Names
OPRD; D-OR-1; DOR-1  [Similar Products]
UniProt Entry Name
OPRD_HUMAN
UniProt Comments for OPRD1
DOR-1: delta opioid receptor, a G-protein coupled receptor. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Highly stereoselective. Receptor for enkephalins.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1

Chromosomal Location of Human Ortholog: 1p36.1-p34.3

Cellular Component: postsynaptic membrane; neuron projection; integral to plasma membrane; cytoplasm; plasma membrane; nerve terminal; intrinsic to plasma membrane; vesicle; lipid raft

Molecular Function: neuropeptide binding; protein binding; opioid receptor activity

Biological Process: adult locomotory behavior; activation of CREB transcription factor; regulation of calcium ion transport; sensory perception of pain; positive regulation of peptidyl-serine phosphorylation; G-protein signaling, coupled to cyclic nucleotide second messenger; negative regulation of protein oligomerization; synaptic transmission; G-protein coupled receptor protein signaling pathway; regulation of mitochondrial membrane potential; protein import into nucleus, translocation; neuropeptide signaling pathway; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); immune response; regulation of sensory perception of pain; G-protein signaling, adenylate cyclase inhibiting pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with OPRD1 elisa kit
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