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P-GSK3beta elisa kit :: Rat Phospho-Glycogen Synthase Kinase 3beta ELISA Kit

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Catalog # MBS730623
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Phospho-Glycogen Synthase Kinase 3beta (P-GSK3beta), ELISA Kit

Popular Item
Also Known As

Rat Phospho-Glycogen Synthase Kinase 3beta ELISA Kit

Product Synonym Names
Rat Phospho-Glycogen Synthase Kinase 3b ELISA Kit
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
604766
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of pGSK3beta. No significant cross-reactivity or interference between pGSK3beta and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between pGSK3beta and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Sandwich
Sensitivity
1.0 pg/mL.
Intended Uses
This pGSK3beta ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat pGSK3beta. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of P-GSK3beta elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forP-GSK3betapurchase
MBS730623 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phospho-Glycogen Synthase Kinase 3beta (P-GSK3beta) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing P-GSK3beta. The ELISA analytical biochemical technique of the MBS730623 kit is based on P-GSK3beta antibody-P-GSK3beta antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect P-GSK3beta antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, P-GSK3beta. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
P-GSK3beta elisa kit
Principle of the assay: pGSK3beta ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for pGSK3beta. Standards or samples are then added to the microtiter plate wells and pGSK3beta if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of pGSK3beta present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for pGSK3beta are added to each well to "sandwich" the pGSK3beta immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain pGSK3beta and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The pGSK3beta concentration in each sample is interpolated from this standard curve.

Typical Testing Data/Standard Curve (for reference only) of P-GSK3beta elisa kit
P-GSK3beta elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS730623. Click to request current manual
NCBI/Uniprot data below describe general gene information for P-GSK3beta. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
34,421 Da
NCBI Official Full Name
podocin isoform 1
NCBI Official Synonym Full Names
nephrosis 2, idiopathic, steroid-resistant (podocin)
NCBI Official Symbol
NPHS2  [Similar Products]
NCBI Official Synonym Symbols
PDCN; SRN1
  [Similar Products]
NCBI Protein Information
podocin
UniProt Protein Name
Podocin
UniProt Gene Name
NPHS2  [Similar Products]
UniProt Entry Name
PODO_HUMAN
NCBI Summary for P-GSK3beta
This gene encodes a protein that plays a role in the regulation of glomerular permeability. Mutations in this gene cause steroid-resistant nephrotic syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
UniProt Comments for P-GSK3beta
NPHS2: Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. Defects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2). It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder. Belongs to the band 7/mec-2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q25.2

Cellular Component: protein complex; integral to plasma membrane; endoplasmic reticulum; plasma membrane; intercellular junction; lipid raft

Molecular Function: protein binding

Biological Process: actin cytoskeleton reorganization; excretion

Disease: Nephrotic Syndrome, Type 2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with P-GSK3beta elisa kit
Organs/Tissues associated with P-GSK3beta elisa kit
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >375 publications with P-GSK3beta and Kidney
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