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anti-P300/CBP antibody :: P300/CBP Antibody

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Catalog # MBS851705
Unit / Price
  0.1 mg  /  $215 +1 FREE 8GB USB
Product Name

P300/CBP, Antibody

Also Known As

P300/CBP Antibody

Product Synonym Names
CREB-binding protein; CREBBP E1A-associated protein p300; EP300; P300 transcription coactivator homolog
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
2414
OMIM
602700
3D Structure
ModBase 3D Structure for Q09472
Species Reactivity
Human, Mouse, Rat
Specificity
P300/CBP antibody detects endogenous levels of total P300/CBP protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized peptide derived from human P300/CBP.
Preparation and Storage
Store at-20 degree C for 1 year
Other Notes
Small volumes of anti-P300/CBP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-P300/CBP antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-P300/CBP antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:10000

Immunohistochemistry (IHC) of anti-P300/CBP antibody
Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using P300/CBP Antibody.
anti-P300/CBP antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-P300/CBP antibody
Western blot analysis of extracts from COLO205 cells, using P300/CBP Antibody.
anti-P300/CBP antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for P300/CBP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
264,161 Da
NCBI Official Full Name
histone acetyltransferase p300 isoform 1
NCBI Official Synonym Full Names
E1A binding protein p300
NCBI Official Symbol
EP300  [Similar Products]
NCBI Official Synonym Symbols
p300; KAT3B; RSTS2
  [Similar Products]
NCBI Protein Information
histone acetyltransferase p300
UniProt Protein Name
Histone acetyltransferase p300
UniProt Synonym Protein Names
E1A-associated protein p300; Histone butyryltransferase p300 (EC:2.3.1.-
UniProt Gene Name
EP300  [Similar Products]
UniProt Synonym Gene Names
P300; p300 HAT  [Similar Products]
NCBI Summary for P300/CBP
This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008]
UniProt Comments for P300/CBP
Functions as histone acetyltransferase and regulates transcription via chromatin remodeling (PubMed:23415232, PubMed:23934153, PubMed:8945521). Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation (PubMed:23415232, PubMed:23934153, PubMed:8945521). Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability. Mediates acetylation of histone H3 at 'Lys-27' (H3K27ac) (PubMed:23911289). Also functions as acetyltransferase for nonhistone targets. Acetylates 'Lys-131' of ALX1 and acts as its coactivator (PubMed:12929931). Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function (PubMed:18722353). Acetylates HDAC1 leading to its inactivation and modulation of transcription (PubMed:16762839). Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2 (PubMed:12586840). Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. Promotes cardiac myocyte enlargement. Can also mediate transcriptional repression. Acetylates FOXO1 and enhances its transcriptional activity (PubMed:15890677). Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity (PubMed:12402037). Participates in CLOCK or NPAS2-regulated rhythmic gene transcription; exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter (PubMed:14645221). Acetylates MTA1 at 'Lys-626' which is essential for its transcriptional coactivator activity (PubMed:16617102). Acetylates XBP1 isoform 2; acetylation increases protein stability of XBP1 isoform 2 and enhances its transcriptional activity (PubMed:20955178). Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER) (PubMed:24939902). Acetylates MEF2D (PubMed:21030595). In addition to protein acetyltransferase, can use different acyl-CoA substrates, such as (2E)-butenoyl-CoA (crotonyl-CoA), butanoyl-CoA (butyryl-CoA) or propanoyl-CoA (propionyl-CoA), and is able to mediate protein crotonylation, butyrylation or propionylation, respectively (PubMed:25818647, PubMed:17267393). Acts as a histone crotonyltransferase; crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors (PubMed:25818647). Histone crotonyltransferase activity is dependent on the concentration of (2E)-butenoyl-CoA (crotonyl-CoA) substrate and such activity is weak when (E)-but-2-enoyl-CoA (crotonyl-CoA) concentration is low (PubMed:25818647). Also acts as a histone butyryltransferase; butyrylation marks active promoters (PubMed:17267393).
Product References and Citations for anti-P300/CBP antibody
Gerd A. Blobel, J. Leukoc. Biol., Apr 2002; 71: 545. Eric Kalkhoven, Hum. Mol. Genet., Feb 2003; 12: 441-450. Wei-fang Shen, Mol. Cell. Biol., Nov 2001; 21: 7509-7522. Frederick C. Nucifora, Science, Mar 2001; 291: 2423.

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