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P38MAPK elisa kit :: Human P 38 Mitogen activated Protein Kinase ELISA Kit

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Catalog # MBS720732
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

P 38 Mitogen activated Protein Kinase (P38MAPK), ELISA Kit

Popular Item
Full Product Name

Human P 38 Mitogen activated Protein Kinase ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of P38MAPK. No significant cross-reactivity or interference between P38MAPK and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between P38MAPK and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
2.5-50ng/mL
Sensitivity
0.1ng/mL
Intended Uses
This P38MAPK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human P38MAPK. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of P38MAPK elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forP38MAPKpurchase
MBS720732 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the P 38 Mitogen activated Protein Kinase (P38MAPK) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing P38MAPK. The ELISA analytical biochemical technique of the MBS720732 kit is based on P38MAPK antibody-P38MAPK antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect P38MAPK antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, P38MAPK. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
P38MAPK elisa kit
Principle of the assay: P38MAPK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-P38MAPK antibody and an P38MAPK-HRP conjugate. The assay sample and buffer are incubated together with P38MAPK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the P38MAPK concentration since P38MAPK from samples and P38MAPK-HRP conjugate compete for the anti-P38MAPK antibody binding site. Since the number of sites is limited, as more sites are occupied by P38MAPK from the sample, fewer sites are left to bind P38MAPK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The P38MAPK concentration in each sample is interpolated from this standard curve.
Product Categories/Family for P38MAPK elisa kit

Typical Testing Data/Standard Curve (for reference only) of P38MAPK elisa kit
P38MAPK elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS720732. Click to request current manual
NCBI/Uniprot data below describe general gene information for P38MAPK. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
p38 map kinase
NCBI Official Synonym Full Names
p38 map kinase
NCBI Official Symbol
P38mapk  [Similar Products]
NCBI Protein Information
p38 map kinase
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with P38MAPK elisa kit
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