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P4HB elisa kit :: Mouse prolyl 4-hydroxylase, beta polypeptide ELISA Kit

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Catalog # MBS933098
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

prolyl 4-hydroxylase, beta polypeptide (P4HB), ELISA Kit

Popular Item
Also Known As

Mouse Protein disulfide-isomerase, P4HB ELISA Kit

Product Synonym Names
Mouse Protein disulfide-isomerase (P4HB) ELISA kit; DSI; ERBA2L; GIT; P4Hbeta; PDI; PDIA1; PHDB; PO4DB; PO4HB; PROHB; collagen prolyl 4-hydroxylase beta; glutathione-insulin transhydrogenase; procollagen-proline; 2-oxoglutarate 4-dioxygenase (proline 4; prolyl 4-hydroxylase; beta polypeptide
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS933098 Testing Data
Sequence Length
509
3D Structure
ModBase 3D Structure for P09103
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse P4HB. No significant cross-reactivity or interference between Mouse P4HB and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Detection Range
0.312 ng/ml-20 ng/ml
Sensitivity
0.078 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of P4HB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forP4HBpurchase
MBS933098 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the prolyl 4-hydroxylase, beta polypeptide (P4HB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing P4HB. The ELISA analytical biochemical technique of the MBS933098 kit is based on P4HB antibody-P4HB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect P4HB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, P4HB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Typical Testing Data/Standard Curve (for reference only) of P4HB elisa kit
P4HB elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS933098. Click to request current manual
NCBI/Uniprot data below describe general gene information for P4HB. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
57,058 Da
NCBI Official Full Name
protein disulfide-isomerase
NCBI Official Synonym Full Names
prolyl 4-hydroxylase, beta polypeptide
NCBI Official Symbol
NCBI Official Synonym Symbols
PDI; Thbp; ERp59; Pdia1
  [Similar Products]
NCBI Protein Information
protein disulfide-isomerase; p55; ER protein 59; protein disulfide isomerase; prolyl 4-hydroxylase subunit beta; cellular thyroid hormone-binding protein; endoplasmic reticulum resident protein 59
UniProt Protein Name
Protein disulfide-isomerase
UniProt Synonym Protein Names
Cellular thyroid hormone-binding protein; Endoplasmic reticulum resident protein 59; ER protein 59; ERp59; Prolyl 4-hydroxylase subunit beta; p55
UniProt Gene Name
UniProt Synonym Gene Names
Pdia1; PDI; ER protein 59; ERp59  [Similar Products]
UniProt Entry Name
PDIA1_MOUSE
UniProt Comments for P4HB
PDIA1: This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Homodimer. Monomers and homotetramers may also occur. Also constitutes the structural subunit of prolyl 4-hydroxylase and of the microsomal triacylglycerol transfer protein MTTP in mammalian cells. Stabilizes both enzymes and retain them in the ER without contributing to the catalytic activity. Binds UBQLN1. Binds to CD4, and upon HIV-1 binding to the cell membrane, is part of a P4HB/PDI-CD4-CXCR4-gp120 complex. Belongs to the protein disulfide isomerase family.

Protein type: Nuclear receptor co-regulator; Oxidoreductase; Endoplasmic reticulum; EC 5.3.4.1; Isomerase

Cellular Component: focal adhesion; membrane; endoplasmic reticulum; plasma membrane; ER-Golgi intermediate compartment

Molecular Function: enzyme binding; procollagen-proline 4-dioxygenase activity; protein heterodimerization activity; protein disulfide isomerase activity; isomerase activity; peptidyl-proline 4-dioxygenase activity

Biological Process: protein folding; cell redox homeostasis; peptidyl-proline hydroxylation to 4-hydroxy-L-proline
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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