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PACAP-27 elisa kit :: Human PACAP-27 ELISA Kit

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Catalog # MBS2510281
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

PACAP-27, ELISA Kit

Popular Item
Also Known As

Human PACAP-27 (Pituitary Adenylate Cyclase Activating Polypeptide 27) ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
447
OMIM
102981
3D Structure
ModBase 3D Structure for P41586
Species Reactivity
Specificity
This kit recognizes natural and recombinant Human PACAP-27. No significant cross-reactivity or interference between Human PACAP-27 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.313-20ng/mL
Sensitivity
Min: 0.188ng/mL; Max: 20ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PACAP-27 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPACAP-27purchase
MBS2510281 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PACAP-27, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PACAP-27. The ELISA analytical biochemical technique of the MBS2510281 kit is based on PACAP-27 antibody-PACAP-27 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PACAP-27 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PACAP-27. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PACAP-27 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human PACAP-27 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PACAP-27. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PACAP-27 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PACAP-27, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PACAP-27. You can calculate the concentration of PACAP-27 in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of PACAP-27 elisa kit
PACAP-27 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2510281. Click to request current manual
NCBI/Uniprot data below describe general gene information for PACAP-27. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
46,957 Da
NCBI Official Full Name
pituitary adenylate cyclase-activating polypeptide type I receptor isoform 4
NCBI Official Synonym Full Names
adenylate cyclase activating polypeptide 1 (pituitary) receptor type I
NCBI Official Symbol
ADCYAP1R1  [Similar Products]
NCBI Official Synonym Symbols
PAC1; PAC1R; PACAPR; PACAPRI
  [Similar Products]
NCBI Protein Information
pituitary adenylate cyclase-activating polypeptide type I receptor; PACAP receptor 1; PACAP type I receptor; PACAP-R1; pituitary adenylate cyclase activating polypeptide 1 receptor type I Hiphop
UniProt Protein Name
Pituitary adenylate cyclase-activating polypeptide type I receptor
UniProt Gene Name
ADCYAP1R1  [Similar Products]
UniProt Synonym Gene Names
PACAP type I receptor; PACAP-R-1; PACAP-R1  [Similar Products]
UniProt Entry Name
PACR_HUMAN
NCBI Summary for PACAP-27
This gene encodes type I adenylate cyclase activating polypeptide receptor, which is a membrane-associated protein and shares significant homology with members of the glucagon/secretin receptor family. This receptor mediates diverse biological actions of adenylate cyclase activating polypeptide 1 and is positively coupled to adenylate cyclase. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Dec 2010]
UniProt Comments for PACAP-27
ADCYAP1R1: This is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract. Belongs to the G-protein coupled receptor 2 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, GPCR; GPCR, family 2; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7p14

Cellular Component: neuron projection; tight junction; cell surface; rough endoplasmic reticulum; integral to plasma membrane; plasma membrane; caveola; endosome; receptor complex

Molecular Function: neuropeptide binding; ADP-ribosylation factor binding; vasoactive intestinal polypeptide receptor activity; protein binding; receptor activity; adenylate cyclase binding

Biological Process: response to drug; development of primary female sexual characteristics; G-protein coupled receptor protein signaling pathway; cAMP-mediated signaling; cell surface receptor linked signal transduction; response to ethanol; nerve growth factor receptor signaling pathway; phospholipase C activation; adenylate cyclase activation; spermatogenesis; cell differentiation; multicellular organismal response to stress; response to estradiol stimulus; transmembrane receptor protein tyrosine kinase signaling pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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