Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Section 5: Information On ELISA And ELISA Sensitivity  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
      • Custom ELISA Kit
      • Avian
      • Bovine/Cow/Cattle
      • Camel
      • Canine/Dog
      • Chicken
      • Donkey
      • Duck
      • Equine/Horse
      • Feline/Cat
      • Fish/Zebrafish
      • Goat/Caprine
      • Goose
      • Guinea Pig/Cavia
      • Hamster
      • Human
      • Monkey
      • Mouse/Murine
      • Non Human Primate
      • Pigeon
      • Plant
      • Porcine/Pig/Swine
      • Primate
      • Rabbit
      • Rat
      • Sheep/Ovine

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • EPSTEIN-BARR VIRUS VCA Antibody
 • SNL Feeder Cells Cells
 • DCK Recombinant Protein
 • Testosterone (T) ELISA Kit
 • Interleukin 17 (IL17) Recombinant Protein
 • NEF3 ELISA Kit
 • Tyrosine Hydroxylase (TH) Antibody
 • Complement 4 (C4) ELISA Kit
 • 7,8-dihydro-8-oxoguanine triphosphatase (Nudt1) Recombinant Protein
 • TRAP Antibody
 • KISS1 ELISA Kit
 • GIT1 Antibody
 • HRP Antibody
 • Serpin Peptidase Inhibitor (SERPINA5) Recombinant Protein
 downarrow more ...
separator

Section 5: Information On ELISA And ELISA Sensitivity

separator
ELISA is essentially a diagnostic tool that is often used in plant pathology and medicine. It is also used in various industries as a tool for quality control. It involves the detection of the specific substance (or analyte) being analyzed quantitatively or qualitatively in a liquid sample. This technique continues to use liquid reagents throughout the process of the evaluation. The reagents remain in its liquid form and stays inside a reaction chamber. They also function to contain the reactants.

ELISA is a heterogenous assay that separates some components of the analytical reaction mixture. Adsorbing particular components onto a solid, physically immobilized phase does this. In ELISA, a solid phase has special binding properties on which a liquid sample is placed. This is followed by the sequential adding of multiple liquid reagents, incubating and washing of the reagents. There will be some optical change of the liquid in the well. The presence of analyte will then be measured from this liquid.

This will generate a qualitative reading typically based on the discovery of the concentration of transmitted light through spectrophotometry. This involves quantifying the transmission of certain specific light wavelength through the liquid. The sensitivity of discovery depends on how the signals are amplified during analytic reactions. Enzyme reactions are well-known amplification processes. Additionally, enzymes produce the signal that is linked to the reagents in fixed proportions. This enables accurate quantification.

ELISA Sensitivity
ELISAs are among the most sensitive immunoassays available. They have a typical detection range between 0.01 ng and 0.1 ng or 0.1 and 1 fmole. ELISA sensitivity relies on the specific characteristics of the interaction between the antibody and the antigen. Additionally, a number of substrates, like the ones yielding fluorescent signal or enhanced chemiluminescent, can be utilized to enhance results. Indirect detection will generate higher levels of signal and as such, should have more sensitivity. However, it can also result in elevated background signal that reduces net specific signal levels.

ELISAs are carried out quickly and easily. They are designed to speedily handle a large amount of parallel samples. Therefore, they are a commonly used to analyze a number of different research and diagnostic targets.
separator
section bar
separator
Introduction Section 1 Section 2
rightarrow An Introduction to ELISA
    - Brief introduction to basic procedures, different types of ELISA tests...
rightarrow Overview Of The Basic ELISA Procedure
    - ELISA procedures, features, benefits, improvements...
rightarrow Understanding The ELISA Assay Types
    - ELISA's advantages, Direct, Sandwich, Competitive ELISA...
separator
Section 3 ↢ Section 4 - Section 5 -
rightarrow Defining ELISA Detection Options
    - The two ELISA detection options: Direct and Indirect...
rightarrow ELISA Results
    - Testing Data: Quantitative, Qualitative, Semi-quantitative...
rightarrow Information On ELISA And ELISA Sensitivity
    - ELISA: The most sensitive immunoassays available...
separator
 Useful Links  rightarrow  About Us :: Contact Us :: Company Frequently Asked Questions
 Page Keywords  rightarrow  Information On ELISA And ELISA Sensitivity; MyBioSource.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions