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PAI-1 recombinant protein

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Catalog # MBS232424
Unit / Price
  0.01 mg  /  $440 +1 FREE 8GB USB
PAI-1 recombinant protein
Product Name

PAI-1, Recombinant Protein

Also Known As

RECOMBINANT HUMAN PAI-1

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
402
OMIM
173360
3D Structure
ModBase 3D Structure for P05121
Purity/Purification
>95% by SDS PAGE and HPLC analysis.
Form/Format
Purified recombinant protein - lyophilised
Concentration
Approximate Protein Concentration: 0.1mg/ml after reconstitution (lot specific)
Reconstitution
Reconstitute with 100ul distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For extended sotrage, the addition of 0.1% BSA is recommended.
Preparation
Buffer Solution
10mM Sodium Phosphate
Activity
Determined by its inhibitory effect against single chain tPA induced cleavage of a chromogenic substrate in Imidazole Buffer at 37 degree C. Half maximal inhibition against 1.0 mug/ml of single chain tPA was obtained at a concentration of 2.0 mug/ml.
Target Species
Human
Preparation and Storage
Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 3 months from date of reconstitution.
Other Notes
Small volumes of PAI-1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PAI-1 recombinant protein
PAI-1 (plasminogen activator inhibitor-1) is a serine protease inhibitor and member of the serpin family, which acts as the primary inhibitor of tissue plasminogen activator (tPA) and urokinase plasminogen activator (uPA), and hence fibrinolysis. Increased levels of PAI-1 are associated with diabetes, cardiovascular disease and an increased occurrence of thrombosis in obesity, and can be induced by obesity related factors including insulin, glucose and VLDL (very-low-density lipoprotein).
Applications Tested/Suitable for PAI-1 recombinant protein
Functional Assays (FN)
NCBI/Uniprot data below describe general gene information for PAI-1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
42.7kD (379 amino acid residues).
NCBI Official Full Name
plasminogen activator inhibitor 1
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
NCBI Official Symbol
SERPINE1  [Similar Products]
NCBI Official Synonym Symbols
PAI; PAI1; PAI-1; PLANH1
  [Similar Products]
NCBI Protein Information
plasminogen activator inhibitor 1; endothelial plasminogen activator inhibitor; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; serpin E1
UniProt Protein Name
Plasminogen activator inhibitor 1
UniProt Synonym Protein Names
Endothelial plasminogen activator inhibitor; Serpin E1
UniProt Gene Name
SERPINE1  [Similar Products]
UniProt Synonym Gene Names
PAI1; PLANH1; PAI; PAI-1  [Similar Products]
UniProt Entry Name
PAI1_HUMAN
NCBI Summary for PAI-1
This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
UniProt Comments for PAI-1
SERPINE1: a secreted protein that acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Belongs to the serpin family. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Plasma levels of PAI-1 and VCAM-1 together may be useful in predicting post-operative recurrence in patients with colorectal cancer.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: extracellular matrix; extracellular space; plasma membrane; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protease binding; receptor binding

Biological Process: circadian rhythm; transcription initiation from RNA polymerase II promoter; platelet activation; extracellular matrix organization and biogenesis; positive regulation of blood coagulation; transcription, DNA-dependent; negative regulation of blood coagulation; negative regulation of smooth muscle cell migration; defense response to Gram-negative bacterium; positive regulation of receptor-mediated endocytosis; regulation of cell proliferation; positive regulation of interleukin-8 production; positive regulation of angiogenesis; fibrinolysis; negative regulation of fibrinolysis; platelet degranulation; transforming growth factor beta receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; gene expression; angiogenesis; regulation of receptor activity; blood coagulation; chronological cell aging; negative regulation of cell adhesion mediated by integrin; negative regulation of cell migration; positive regulation of inflammatory response

Disease: Plasminogen Activator Inhibitor-1 Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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