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PCMT1 recombinant protein :: Protein-L-Isoaspartate O-Methyltransferase Recombinant Protein

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Catalog # MBS144034
Unit / Price
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  0.005 mg  /  $140 +1 FREE 8GB USB
  0.025 mg  /  $205 +1 FREE 8GB USB
  1 mg  /  $2,235 +2 FREE 8GB USB
PCMT1 recombinant protein
Product Name

Protein-L-Isoaspartate O-Methyltransferase (PCMT1), Recombinant Protein

Popular Item
Full Product Name

Recombinant Human Protein-L-Isoaspartate O-Methyltransferase

Product Synonym Names
PCMT1 Human; Protein-L-Isoaspartate O-Methyltransferase Human Recombinant; EC 2.1.1.77; PIMT; PCMT1; Protein-beta-aspartate methyltransferase; Protein L-isoaspartyl/D-aspartyl methyltransferase; L-isoaspartyl protein carboxyl methyltransferase
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
RGSHHHH HH GMASMTGGQQ MGRDLYDDDD KDRWGS MAWK SGGASHSELI HNLRKNGIIK TDKVFEVMLA TDRSHYAKCN PYMDSPQSIG FQATISAPHM HAYALELLFD QLHEGAKALD VGSGSGILTA CFARMVGCTG KVIGIDHIKE LVDDSINNVR KDDPTLLSSG RVQLVVGDGR MGYAEEAPYD AIHVGAAAPV VPQALIDQLK PGGRLILPVG PAGGNQMLEQ YDKLQDGSIK MKPLMGVIYV PLTDKEKQWS RWK
OMIM
176851
3D Structure
ModBase 3D Structure for P22061
Purity/Purification
Greater than 95.0% as determined by SDS-PAGE.
Form/Format
PCMT1 Human solution containing 20mM Tris-HCl pH-8, 0.1M NaCl, & 10% glycerol.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Preparation and Storage
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Other Notes
Small volumes of PCMT1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PCMT1 recombinant protein
Description: PCMT1 Recombinant Human produced in E Coli is a single, non-glycosylated polypeptide chain containing 263 amino acids (1-227 a.a.) and having a molecular mass of 28.8 kDa. The PCMT1 is fused to 36 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Introduction: PCMT1 enzyme catalyses the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. PCMT1 is involved in the repair and/or degradation of damaged proteins.
NCBI/Uniprot data below describe general gene information for PCMT1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
24,679 Da
NCBI Official Full Name
protein-L-isoaspartate(D-aspartate) O-methyltransferase isoform 2
NCBI Official Synonym Full Names
protein-L-isoaspartate (D-aspartate) O-methyltransferase
NCBI Official Symbol
PCMT1  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
protein-L-isoaspartate(D-aspartate) O-methyltransferase; protein-L-isoaspartate(D-aspartate) O-methyltransferase; L-isoaspartyl protein carboxyl methyltransferase; protein L-isoaspartyl/D-aspartyl methyltransferase; protein-beta-aspartate methyltransferase
UniProt Protein Name
Protein-L-isoaspartate(D-aspartate) O-methyltransferase
UniProt Synonym Protein Names
L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl/D-aspartyl methyltransferase; Protein-beta-aspartate methyltransferase
UniProt Gene Name
PCMT1  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
PIMT_HUMAN
NCBI Summary for PCMT1
This gene encodes a member of the type II class of protein carboxyl methyltransferase enzymes. The encoded enzyme plays a role in protein repair by recognizing and converting D-aspartyl and L-isoaspartyl residues resulting from spontaneous deamidation back to the normal L-aspartyl form. The encoded protein may play a protective role in the pathogenesis of Alzheimer's disease, and single nucleotide polymorphisms in this gene have been associated with spina bifida and premature ovarian failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]
UniProt Comments for PCMT1
PCMT1: Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl- terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein. Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.77; Methyltransferase

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: endoplasmic reticulum; cytoplasm; vesicle

Molecular Function: protein-L-isoaspartate (D-aspartate) O-methyltransferase activity

Biological Process: protein repair; protein amino acid methylation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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