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PCPE1 elisa kit :: Mouse Procollagen C Proteinase Enhancer 1 ELISA Kit

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Catalog # MBS724700
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Procollagen C Proteinase Enhancer 1 (PCPE1), ELISA Kit

Popular Item
Also Known As

Mouse Procollagen C Proteinase Enhancer 1 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
600270
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of PCPE1. No significant cross-reactivity or interference between PCPE1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PCPE1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
1.0-25ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PCPE1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPCPE1purchase
MBS724700 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Procollagen C Proteinase Enhancer 1 (PCPE1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PCPE1. The ELISA analytical biochemical technique of the MBS724700 kit is based on PCPE1 antibody-PCPE1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PCPE1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PCPE1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PCPE1 elisa kit
Principle of the assay: PCPE1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PCPE1 antibody and an PCPE1-HRP conjugate. The assay sample and buffer are incubated together with PCPE1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PCPE1 concentration since PCPE1 from samples and PCPE1-HRP conjugate compete for the anti-PCPE1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PCPE1 from the sample, fewer sites are left to bind PCPE1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PCPE1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for PCPE1 elisa kit

Typical Testing Data/Standard Curve (for reference only) of PCPE1 elisa kit
PCPE1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS724700. Click to request current manual
NCBI/Uniprot data below describe general gene information for PCPE1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
47,972 Da
NCBI Official Full Name
procollagen C-endopeptidase enhancer 1
NCBI Official Synonym Full Names
procollagen C-endopeptidase enhancer
NCBI Official Symbol
PCOLCE  [Similar Products]
NCBI Official Synonym Symbols
PCPE; PCPE1; PCPE-1
  [Similar Products]
NCBI Protein Information
procollagen C-endopeptidase enhancer 1; procollagen C-proteinase enhancer 1; procollagen COOH-terminal proteinase enhancer 1; type 1 procollagen C-proteinase enhancer protein; type I procollagen COOH-terminal proteinase enhancer; procollagen, type 1, COOH-terminal proteinase enhancer
UniProt Protein Name
Procollagen C-endopeptidase enhancer 1
UniProt Synonym Protein Names
Procollagen COOH-terminal proteinase enhancer 1; PCPE-1; Procollagen C-proteinase enhancer 1; Type 1 procollagen C-proteinase enhancer protein; Type I procollagen COOH-terminal proteinase enhancer
UniProt Gene Name
PCOLCE  [Similar Products]
UniProt Synonym Gene Names
PCPE1; PCPE-1; Procollagen C-proteinase enhancer 1  [Similar Products]
UniProt Entry Name
PCOC1_HUMAN
NCBI Summary for PCPE1
Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity. [provided by RefSeq, Jul 2008]
UniProt Comments for PCPE1
PCOLCE: Binds to the C-terminal propeptide of type I procollagen and enhances procollagen C-proteinase activity.

Protein type: Secreted, signal peptide; Extracellular matrix; Secreted

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: extracellular matrix; extracellular space

Molecular Function: heparin binding; collagen binding; protein binding; protease activator activity

Biological Process: multicellular organismal development; proteolysis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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