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PDAP1 elisa kit :: Mouse PDAP1 ELISA Kit

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Catalog # MBS2503979
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

PDAP1, ELISA Kit

Popular Item
Also Known As

Mouse PDAP1 (PDGFA Associated Protein 1) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
180
3D Structure
ModBase 3D Structure for Q3UHX2
Species Reactivity
Specificity
This kit recognizes natural and recombinant Mouse PDAP1. No significant cross-reactivity or interference between Mouse PDAP1 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.313-20ng/mL
Sensitivity
Min: 0.188ng/mL; Max: 20ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PDAP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPDAP1purchase
MBS2503979 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PDAP1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PDAP1. The ELISA analytical biochemical technique of the MBS2503979 kit is based on PDAP1 antibody-PDAP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PDAP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PDAP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PDAP1 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse PDAP1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PDAP1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PDAP1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PDAP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PDAP1. You can calculate the concentration of PDAP1 in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of PDAP1 elisa kit
PDAP1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2503979. Click to request current manual
NCBI/Uniprot data below describe general gene information for PDAP1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
20,605 Da
NCBI Official Full Name
Pdap1 protein, partial
NCBI Official Synonym Full Names
PDGFA associated protein 1
NCBI Official Symbol
Pdap1  [Similar Products]
NCBI Official Synonym Symbols
PAP; PAP1; HASPP28
  [Similar Products]
NCBI Protein Information
28 kDa heat- and acid-stable phosphoprotein; PDGF-associated protein; PDGFA-associated protein 1
UniProt Protein Name
28 kDa heat- and acid-stable phosphoprotein
UniProt Synonym Protein Names
PDGF-associated protein; PAP; PDGFA-associated protein 1; PAP1
UniProt Gene Name
Pdap1  [Similar Products]
UniProt Synonym Gene Names
PAP; PAP1  [Similar Products]
UniProt Entry Name
HAP28_MOUSE
UniProt Comments for PDAP1
PDAP1: Enhances PDGFA-stimulated cell growth in fibroblasts, but inhibits the mitogenic effect of PDGFB. Belongs to the PDAP1 family.

Cellular Component: extracellular space

Molecular Function: platelet-derived growth factor binding

Biological Process: platelet-derived growth factor receptor signaling pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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