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PDCD4 elisa kit :: Human programmed cell death 4 (neoplastic transformation inhibitor) ELISA Kit

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Catalog # MBS941857
Unit / Price
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Typical Testing Data/Standard Curve (for reference only)
Product Name

programmed cell death 4 (neoplastic transformation inhibitor) (PDCD4), ELISA Kit

Popular Item
Also Known As

Human programmed cell death 4, PDCD4 ELISA Kit

Product Synonym Names
Human programmed cell death 4 (PDCD4) ELISA Kit; RP11-348N5.4; H731; MGC33046; MGC33047; nuclear antigen H731; programmed cell death 4; programmed cell death 4 (neoplastic transformation inhibitor)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS941857 Testing Data
Sequence Length
469
OMIM
608610
3D Structure
ModBase 3D Structure for Q53EL6
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human PDCD4. No significant cross-reactivity or interference between human PDCD4 and analogues was observed.
Samples
serum, plasma
Assay Type
Quantitative Sandwich
Detection Range
0.156 ng/ml -10 ng/ml
Sensitivity
<0.039 ng/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PDCD4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPDCD4purchase
MBS941857 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the programmed cell death 4 (neoplastic transformation inhibitor) (PDCD4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PDCD4. The ELISA analytical biochemical technique of the MBS941857 kit is based on PDCD4 antibody-PDCD4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PDCD4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PDCD4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PDCD4 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PDCD4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDCD4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDCD4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDCD4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of PDCD4 elisa kit
PDCD4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS941857. Click to request current manual
NCBI/Uniprot data below describe general gene information for PDCD4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
51,735 Da
NCBI Official Full Name
programmed cell death protein 4 isoform 1
NCBI Official Synonym Full Names
programmed cell death 4 (neoplastic transformation inhibitor)
NCBI Official Symbol
PDCD4  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
programmed cell death protein 4; protein 197/15a; nuclear antigen H731; neoplastic transformation inhibitor protein
UniProt Protein Name
Programmed cell death protein 4
UniProt Synonym Protein Names
Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; Protein 197/15a
UniProt Gene Name
PDCD4  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
PDCD4_HUMAN
NCBI Summary for PDCD4
This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]
UniProt Comments for PDCD4
PDCD4: protein localized to the nucleus in proliferating cells that seems to possess a tumor suppressor activity. Directly interacts with the RNA helicase eIF4A and inhibits protein synthesis by interfering with the assembly of the cap-dependent translation initiation complex. Suppresses carbonic anhydrase type II protein expression in carcinoid cell lines. Since tumor cells require a high bicarbonate flux for their growth, carbonic anhydrase suppression results in growth inhibition. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two differentially spliced isoforms have been identified.

Protein type: Apoptosis; Tumor suppressor

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; RNA binding

Biological Process: negative regulation of JNK activity; apoptosis; cell aging; negative regulation of transcription, DNA-dependent; negative regulation of cell cycle
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with PDCD4 elisa kit
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