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PDCN elisa kit :: Rat Podocin (PDCN) ELISA Kit

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Catalog # MBS2019246 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  24-Strip-Wells  /  $265 +1 FREE 8GB USB
  48-Strip-Wells  /  $490 +1 FREE 8GB USB
  96-Strip-Wells  /  $655 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,660 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,795 +5 FREE 8GB USB
Typical Testing Data/Standard Curve
Product Name

Podocin (PDCN), ELISA Kit

Popular Item
Also Known As

Podocin (PDCN) ELISA Kit

Product Synonym Names
NPHS2; NPHS-2; Nephrosis 2; Idiopathic Steroid-Resistant
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
383
OMIM
600995
3D Structure
ModBase 3D Structure for Q9NP85
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Podocin (PDCN).
No significant cross-reactivity or interference between Podocin (PDCN) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Urine, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.056ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Podocin (PDCN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Podocin (PDCN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PDCN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPDCNpurchase
MBS2019246 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Podocin (PDCN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PDCN. The ELISA analytical biochemical technique of the MBS2019246 kit is based on PDCN antibody-PDCN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PDCN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PDCN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PDCN elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Podocin (PDCN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Podocin (PDCN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Podocin (PDCN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Podocin (PDCN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve of PDCN elisa kit
PDCN elisa kit Typical Testing Data/Standard Curve image
Sample Manual Insert of MBS2019246. Click to request current manual
NCBI/Uniprot data below describe general gene information for PDCN. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
34,421 Da
NCBI Official Full Name
podocin isoform 1
NCBI Official Synonym Full Names
nephrosis 2, idiopathic, steroid-resistant (podocin)
NCBI Official Symbol
NPHS2  [Similar Products]
NCBI Official Synonym Symbols
PDCN; SRN1
  [Similar Products]
NCBI Protein Information
podocin
UniProt Protein Name
Podocin
UniProt Gene Name
NPHS2  [Similar Products]
UniProt Entry Name
PODO_HUMAN
NCBI Summary for PDCN
This gene encodes a protein that plays a role in the regulation of glomerular permeability. Mutations in this gene cause steroid-resistant nephrotic syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
UniProt Comments for PDCN
NPHS2: Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. Defects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2). It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder. Belongs to the band 7/mec-2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q25.2

Cellular Component: protein complex; endoplasmic reticulum; integral to plasma membrane; plasma membrane; intercellular junction; lipid raft

Molecular Function: protein binding

Biological Process: actin cytoskeleton reorganization; excretion

Disease: Nephrotic Syndrome, Type 2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with PDCN elisa kit
Organs/Tissues associated with PDCN elisa kit
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >387 publications with PDCN and Kidney
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