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PDE1 enzyme :: Phosphodiesterase 1 Enzyme

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Catalog # MBS653995
Unit / Price
  100 Units  /  $375 +1 FREE 8GB USB
PDE1 enzyme
Product Name

Phosphodiesterase 1 (PDE1), Enzyme

Popular Item
Full Product Name

Phosphodiesterase 1

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
chromosome: Un
Host
Crotalus adamanteus venom (Eastern Diamondback rattlesnake)
Purity/Purification
Molecular Biology Grade
Form/Format
Supplied as a lyophilized powder
Activity
90.3 u/mg dry weight, 102 u/vial
Absorbance (A280)
0.97
Nucleotidase
0.76%
Optimum pH
9.8-10.4
Inhibitors
Reducing agents such as glutathione, cysteine and ascorbic acid. It is completely inhibited by 5mM EDTA while ATP, ADP and AMP are partial inhibitors.
Activators
The enzyme has an absolute requirement for Mg2+ indicates an optimum concentration of 15mM.
Unit Definition
1 unit hydrolyzes 1 umole of p-nitrophenyl thymidine-5-phosphate per minute at 25ºC, pH 8.9.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 6 months after receipt at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of PDE1 enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PDE1 enzyme
Venom exonuclease (Phosphodiesterase I) successively hydrolyzes 5'-mononucleotides from 3'-hydroxy-terminated ribo-and deoxyribo-oligonucleotides. The enzyme has been widely utilized as a tool for structural and sequence studies of nucleic acids. The enzyme has been purified with endonuclease activity being eliminated as well as 5'-nucleotidase and nonspecific monophosphatase. It is nonspecific with respect to base or sugar moieties of nucleotides. A variety of synthetic substrates are hydrolyzed. The exonuclease will not recognize nucleoside units in the syn conformation. ADP-ribosylated proteins are cleaved at the pyrophosphate linkages by venom phosphodiesterase to yield phosphoribosyl-AMP.
NCBI/Uniprot data below describe general gene information for PDE1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
NCBI Official Full Name
phosphodiesterase 1
NCBI Official Symbol
NCBI Protein Information
phosphodiesterase 1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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