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PDE3B elisa kit :: Human Phosphodiesterase 3B, cGMP Inhibited (PDE3B) ELISA Kit

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Catalog # MBS457126
Unit / Price
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 Typical Testing Data/Standard Curve (for reference only)
Product Name

Phosphodiesterase 3B, cGMP Inhibited (PDE3B), ELISA Kit

Full Product Name

Human Phosphodiesterase 3B, cGMP Inhibited (PDE3B) ELISA Kit

Product Synonym Names
HcGIP1; cGIPDE1; CGIPDE1; CGIP1; CGI-PDE B; Cyclic GMP-inhibited phosphodiesterase B
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
U38178 Genomic DNA
3D Structure
ModBase 3D Structure for Q13370
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of PDE3B. No significant cross-reactivity or interference between PDE3B and analogues was observed.
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
78.125-5000pg/mL
Sensitivity
<26.35pg/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PDE3B were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PDE3B were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PDE3B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for PDE3Bpurchase
MBS457126 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phosphodiesterase 3B, cGMP Inhibited (PDE3B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PDE3B. The ELISA analytical biochemical technique of the MBS457126 kit is based on PDE3B antibody-PDE3B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PDE3B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PDE3B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PDE3B elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PDE3B in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDE3B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PDE3B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PDE3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PDE3B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of PDE3B elisa kit
PDE3B elisa kit  Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS457126. Click to request current manual
NCBI/Uniprot data below describe general gene information for PDE3B. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
118,809 Da
NCBI Official Full Name
cGMP-inhibited 3',5'-cyclic phosphodiesterase B
NCBI Official Synonym Full Names
phosphodiesterase 3B
NCBI Official Symbol
PDE3B  [Similar Products]
NCBI Official Synonym Symbols
HcGIP1; cGIPDE1
  [Similar Products]
NCBI Protein Information
cGMP-inhibited 3',5'-cyclic phosphodiesterase B
UniProt Protein Name
cGMP-inhibited 3',5'-cyclic phosphodiesterase B
UniProt Synonym Protein Names
CGIPDE1; CGIP1; Cyclic GMP-inhibited phosphodiesterase B; CGI-PDE B
UniProt Gene Name
PDE3B  [Similar Products]
UniProt Synonym Gene Names
CGIP1; CGI-PDE B  [Similar Products]
UniProt Comments for PDE3B
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism. Regulates cAMP binding of RAPGEF3. Through simultaneous binding to RAPGEF3 and PIK3R6 assembles a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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