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PDGF AA recombinant protein :: Platelet Derived Growth Factor-AA Recombinant Protein

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Catalog # MBS142218
Unit / Price
  0.002 mg  /  $140 +1 FREE 8GB USB
  0.01 mg  /  $205 +1 FREE 8GB USB
  1 mg  /  $3,625 +4 FREE 8GB USB
PDGF AA recombinant protein
Product Name

Platelet Derived Growth Factor-AA (PDGF AA), Recombinant Protein

Also Known As

Recombinant Human Platelet Derived Growth Factor-AA

Product Synonym Names
PDGF AA Human; Platelet-Derived Growth Factor AA Human Recombinant; Glioma-derived growth factor; GDGF; Osteosarcoma-derived Growth Factor; ODGF; PDGF-AA; PDGF-1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
211
Sequence
SIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATTSLNP DYREEDTGRP RESGKKRKRK RLKPT
OMIM
173430
3D Structure
ModBase 3D Structure for P04085
Host
E Coli
Purity/Purification
Greater than 95.0% as determined by SDS-PAGE.
Form/Format
Lyophilized without any additives.
Sterile Filtered White lyophilized (freeze-dried) powder.
Solubility
It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-AA in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity
The ED50, calculated by the dose-dependent proliferation of murine 3T3 indicator cells is < 0.32ng/ml, corresponding to a Specific Activity of 3,125x103units/mg.
Preparation and Storage
Lyophilized Platelet-Derived Growth Factor AA although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution PDGF-AA should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Other Notes
Small volumes of PDGF AA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PDGF AA recombinant protein
Description: Platelet-Derived Growth Factor AA Human Recombinant is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 125 amino acids and having a total molecular mass of 28511 Dalton. PDGF-AA is purified by proprietary chromatographic techniques.

Introduction: PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGF is stored in platelet alpha-granules and released upon platelet activation. The PDGF is involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGF have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB.
NCBI/Uniprot data below describe general gene information for PDGF AA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
22,253 Da
NCBI Official Full Name
platelet-derived growth factor subunit A isoform 1 preproprotein
NCBI Official Synonym Full Names
platelet-derived growth factor alpha polypeptide
NCBI Official Symbol
PDGFA  [Similar Products]
NCBI Official Synonym Symbols
PDGF1; PDGF-A
  [Similar Products]
NCBI Protein Information
platelet-derived growth factor subunit A; PDGF A-chain; PDGF subunit A; PDGF-1; platelet-derived growth factor A chain; platelet-derived growth factor alpha chain; platelet-derived growth factor alpha isoform 2 preproprotein
UniProt Protein Name
Platelet-derived growth factor subunit A
UniProt Synonym Protein Names
PDGF-1; Platelet-derived growth factor A chain; Platelet-derived growth factor alpha polypeptide
UniProt Gene Name
PDGFA  [Similar Products]
UniProt Synonym Gene Names
PDGF1; PDGF subunit A  [Similar Products]
UniProt Entry Name
PDGFA_HUMAN
NCBI Summary for PDGF AA
The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; knockout mice die either as embryos or shortly after birth. Two splice variants have been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for PDGF AA
PDGFA: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Cell cycle regulation; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: Golgi membrane; extracellular space; cell surface; microvillus; endoplasmic reticulum lumen; extracellular region

Molecular Function: collagen binding; protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding

Biological Process: skin development; extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; wound healing; negative chemotaxis; platelet-derived growth factor receptor signaling pathway; cell projection biogenesis; response to estradiol stimulus; positive regulation of MAP kinase activity; positive regulation of fibroblast proliferation; platelet degranulation; regulation of smooth muscle cell migration; hair follicle development; cell-cell signaling; positive regulation of MAPKKK cascade; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; response to wounding; angiogenesis; regulation of peptidyl-tyrosine phosphorylation; inner ear development; response to drug; epidermal growth factor receptor signaling pathway; platelet activation; fibroblast growth factor receptor signaling pathway; response to retinoic acid; response to inorganic substance; phosphoinositide-mediated signaling; negative regulation of phosphatidylinositol biosynthetic process; positive regulation of protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase cascade; cell activation; positive regulation of protein kinase B signaling cascade; organ morphogenesis; regulation of actin cytoskeleton organization and biogenesis; positive regulation of cell division; response to hypoxia; innate immune response; blood coagulation; actin cytoskeleton organization and biogenesis; alveolus development; positive regulation of DNA replication; positive regulation of cell migration
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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