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PDGF-BB recombinant protein

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Catalog # MBS355607
Unit / Price
  0.01 mg  /  $245 +1 FREE 8GB USB
  0.1 mg  /  $410 +1 FREE 8GB USB
PDGF-BB recombinant protein
Product Name

PDGF-BB, Recombinant Protein

Popular Item
Also Known As

PDGF-BB Protein (Active)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 22; NC_000022.10 (39619685..39640957, complement). Location: 22q13.1
OMIM
190040
3D Structure
ModBase 3D Structure for P01127
Host
Pichia yeast
Form/Format
Lyophilized
Biological Activity
The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is < 1 ng/ml corresponding to a specific activity of 1 × 106 IU/mg.
Dimers
< 1% by SDS-PAGE
Endotoxin Level
< 0.1 ng/ug (1 EU/ug) by LAL test
Reconstitution
Reconstitute with sterile 18MΩ-cm H2O.
Storage Buffer
Each vial contains 10 mM Phosphate buffer and 200 mM NaCl (pH7.0) without preservatives.
Preparation and Storage
Ship at 4 degree C. Upon receipt, store at -20 degree C or -80 degree C for 2 years. After reconstituting, it can store at 4 degree C for a week, it is recommended to add 0.1% HSA or BSA for long term. Avoid repeated freeze and thaw cycles
Other Notes
Small volumes of PDGF-BB recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PDGF-BB recombinant protein
Recombinant human PDGF-BB protein with molecular weight 28 kDa.
Applications Tested/Suitable for PDGF-BB recombinant protein
HPLC, SDS-PAGE
NCBI/Uniprot data below describe general gene information for PDGF-BB. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
27,283 Da
NCBI Official Full Name
platelet-derived growth factor subunit B isoform 2 preproprotein
NCBI Official Synonym Full Names
platelet-derived growth factor beta polypeptide
NCBI Official Symbol
PDGFB  [Similar Products]
NCBI Official Synonym Symbols
SIS; SSV; PDGF2; c-sis; PDGF-2
  [Similar Products]
NCBI Protein Information
platelet-derived growth factor subunit B; becaplermin; PDGF, B chain; PDGF subunit B; proto-oncogene c-Sis; platelet-derived growth factor 2; platelet-derived growth factor B chain; platelet-derived growth factor, beta polypeptide (oncogene SIS); platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
UniProt Protein Name
Platelet-derived growth factor subunit B
UniProt Synonym Protein Names
PDGF-2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Proto-oncogene c-Sis
UniProt Gene Name
PDGFB  [Similar Products]
UniProt Synonym Gene Names
PDGF2; SIS; PDGF subunit B  [Similar Products]
UniProt Entry Name
PDGFB_HUMAN
NCBI Summary for PDGF-BB
The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2013]
UniProt Comments for PDGF-BB
Function: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA

By similarity.

Subunit structure: Homodimer; antiparallel disulfide-linked dimer. Heterodimer with PDGFA; antiparallel disulfide-linked dimer. The PDGFB homodimer interacts with PDGFRA and PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. The heterodimer composed of PDGFA and PDGFB interacts with PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. Interacts with XLKD1

By similarity. Ref.19 Ref.24

Subcellular location: Secreted. Note: Released by platelets upon wounding.

Tissue specificity: Expressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung. Ref.20

Involvement in disease: A chromosomal aberration involving PDGFB is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGFB. Ref.21

Pharmaceutical use: Available under the name Regranex (Ortho-McNeil). Used to promote healing in diabetic neuropathic foot ulcers.

Sequence similarities: Belongs to the PDGF/VEGF growth factor family.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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