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PDGF-D elisa kit :: Rat PDGF-D ELISA Kit

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Catalog # MBS2504978
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

PDGF-D, ELISA Kit

Popular Item
Also Known As

Rat PDGF-D (Platelet Derived Growth Factor D) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
364
OMIM
609673
3D Structure
ModBase 3D Structure for Q9GZP0
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat PDGF-D. No significant cross-reactivity or interference between Rat PDGF-D and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rat PDGF-D and all the analogues, therefore, cross reaction may still exist.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.313-20ng/mL
Sensitivity
Min: 0.188ng/mL; Max: 20ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PDGF-D elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPDGF-Dpurchase
MBS2504978 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PDGF-D, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PDGF-D. The ELISA analytical biochemical technique of the MBS2504978 kit is based on PDGF-D antibody-PDGF-D antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PDGF-D antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PDGF-D. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PDGF-D elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PDGF-D in Rat serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PDGF-D. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat PDGF-D and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PDGF-D, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat PDGF-D. You can calculate the concentration of Rat PDGF-D in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of PDGF-D elisa kit
PDGF-D elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2504978. Click to request current manual
NCBI/Uniprot data below describe general gene information for PDGF-D. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
42,167 Da
NCBI Official Full Name
platelet-derived growth factor D isoform 2
NCBI Official Synonym Full Names
platelet derived growth factor D
NCBI Official Symbol
PDGFD  [Similar Products]
NCBI Official Synonym Symbols
IEGF; SCDGFB; MSTP036; SCDGF-B
  [Similar Products]
NCBI Protein Information
platelet-derived growth factor D; PDGF-D; iris-expressed growth factor; spinal cord derived growth factor B; spinal cord-derived growth factor B; spinal cord-derived growth factor-B
UniProt Protein Name
Platelet-derived growth factor D
UniProt Synonym Protein Names
Iris-expressed growth factor; Spinal cord-derived growth factor B; SCDGF-B
UniProt Gene Name
PDGFD  [Similar Products]
UniProt Synonym Gene Names
IEGF; SCDGFB; PDGF-D; SCDGF-B; PDGFD latent form; PDGFD receptor-binding form  [Similar Products]
UniProt Entry Name
PDGFD_HUMAN
NCBI Summary for PDGF-D
The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. Two splice variants have been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for PDGF-D
PDGFD: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Plays an important role in wound healing. Induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. Can initiate events that lead to a mesangial proliferative glomerulonephritis, including influx of monocytes and macrophages and production of extracellular matrix. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: Golgi membrane; endoplasmic reticulum lumen; extracellular region

Molecular Function: growth factor activity; platelet-derived growth factor receptor binding

Biological Process: positive regulation of cell division; multicellular organismal development; platelet-derived growth factor receptor signaling pathway; regulation of peptidyl-tyrosine phosphorylation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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