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PDGFB,PDGFA elisa kit :: Mouse PDGF-AB ELISA Kit

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Catalog # MBS175775
Unit / Price
  96-Strip-Wells  /  $455 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

PDGF-AB (PDGFB,PDGFA), ELISA Kit

Also Known As

Mouse PDGF-AB PicoKine ELISA Kit

Product Synonym Names
Platelet-derived growth factor subunit B,Platelet-derived growth factor subunit A; platelet-derived growth factor beta polypeptide,platelet-derived growth factor alpha polypeptide; PDGF A chain; PDGF B chain; PDGF1; PDGF2; PDGFA; PDGFB; Platelet derived growth factor AB heterodimer
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
241
3D Structure
ModBase 3D Structure for P31240
Species Reactivity
Specificity
Natural and recombinant mouse PDGF-AB
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
< 3pg/ml
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PDGFB,PDGFA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPDGFB,PDGFApurchase
MBS175775 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PDGF-AB (PDGFB,PDGFA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PDGFB,PDGFA. The ELISA analytical biochemical technique of the MBS175775 kit is based on PDGFB,PDGFA antibody-PDGFB,PDGFA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PDGFB,PDGFA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PDGFB,PDGFA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PDGFB,PDGFA elisa kit
Principle of the assay: mouse PDGF-AB ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for PDGF-AB has been precoated onto 96-well plates. Standards (E.coli,(S87-T211)+(S82-T190)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PDGF-AB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse PDGF-AB amount of sample captured in plate.
Background: The platelet-derived growth factor (PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains.1 The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor areexpressed in human malignant glioma cell lines.2 Normal human endothelial cells in culture express the B chain ofPDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281.3 The entire B chain of PDGF is highly (96%) homologous to a portion of p28sis, the transforming protein ofsimian sarcoma virus (SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner.1 PDGF A-chain precursorpolypeptide is assigned to the proximal long arm of chromosome 7, band q11.23.4 The human homolog (PDGFB-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22.5

Typical Testing Data/Standard Curve (for reference only) of PDGFB,PDGFA elisa kit
Mouse PDGF-AB PicoKine ELISA Kit standard curve
PDGFB,PDGFA elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS175775. Click to request current manual
NCBI/Uniprot data below describe general gene information for PDGFB,PDGFA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
27,382 Da
NCBI Official Full Name
Platelet-derived growth factor subunit B
NCBI Official Synonym Full Names
platelet derived growth factor, B polypeptide
NCBI Official Symbol
Pdgfb  [Similar Products]
NCBI Official Synonym Symbols
Sis; PDGF-B
  [Similar Products]
NCBI Protein Information
platelet-derived growth factor subunit B; c-sis; PDGF-2; PDGF subunit B; proto-oncogene c-Sis; platelet-derived growth factor B chain; platelet-derived growth factor beta polypeptide
UniProt Protein Name
Platelet-derived growth factor subunit B
UniProt Synonym Protein Names
PDGF-2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Proto-oncogene c-Sis
UniProt Gene Name
Pdgfb  [Similar Products]
UniProt Synonym Gene Names
Sis; PDGF subunit B  [Similar Products]
UniProt Entry Name
PDGFB_MOUSE
UniProt Comments for PDGFB,PDGFA
PDGFB: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA. A chromosomal aberration involving PDGFB is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGFB. Belongs to the PDGF/VEGF growth factor family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Oncoprotein; Secreted

Cellular Component: extracellular space; cell surface; membrane; basolateral plasma membrane; cytoplasm; extracellular region

Molecular Function: collagen binding; identical protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding; superoxide-generating NADPH oxidase activator activity; chemoattractant activity; receptor binding

Biological Process: embryonic placenta development; peptidyl-tyrosine phosphorylation; positive regulation of cyclin-dependent protein kinase activity; positive regulation of transcription, DNA-dependent; heart development; multicellular organismal development; positive regulation of smooth muscle cell proliferation; positive regulation of fibroblast growth factor receptor signaling pathway; platelet-derived growth factor receptor signaling pathway; cell projection biogenesis; positive regulation of smooth muscle cell migration; protein amino acid phosphorylation; monocyte chemotaxis; positive regulation of MAP kinase activity; positive regulation of fibroblast proliferation; negative regulation of vasodilation; positive chemotaxis; positive regulation of MAPKKK cascade; positive regulation of glomerular filtration; positive regulation of cell proliferation; response to wounding; positive regulation of mitotic cell cycle, embryonic; negative regulation of cell migration; regulation of peptidyl-tyrosine phosphorylation; substrate-bound cell migration; positive regulation of mitosis; positive regulation of chemotaxis; activation of protein kinase B; negative regulation of phosphatidylinositol biosynthetic process; positive regulation of blood vessel endothelial cell migration; positive regulation of phosphoinositide 3-kinase activity; positive regulation of protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; peptidyl-serine phosphorylation; positive regulation of cell division; positive regulation of vasoconstriction; activation of protein kinase activity; blood vessel morphogenesis; positive regulation of endothelial cell proliferation; negative regulation of transcription, DNA-dependent; blood coagulation; actin cytoskeleton organization and biogenesis; positive regulation of DNA replication; positive regulation of cell migration
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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