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Pdk4 recombinant protein :: [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial (Pdk4), partial Recombinant Protein

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Catalog # MBS1216721
Unit / Price
  0.5 mg (E-Coli)  /  $1,015 +1 FREE 8GB USB
  0.05 mg (Baculovirus)  /  $1,015 +1 FREE 8GB USB
  0.05 mg (Mammalian-Cell)  /  $1,240 +1 FREE 8GB USB
  0.5 mg (Yeast)  /  $1,240 +1 FREE 8GB USB
  1 mg (E-Coli)  /  $1,510 +1 FREE 8GB USB
  0.1 mg (Baculovirus)  /  $1,510 +1 FREE 8GB USB
  0.1 mg (Mammalian-Cell)  /  $1,970 +2 FREE 8GB USB
  1 mg (Yeast)  /  $1,970 +2 FREE 8GB USB
  0.5 mg (Baculovirus)  /  $2,005 +2 FREE 8GB USB
  1 mg (Baculovirus)  /  $2,970 +3 FREE 8GB USB
SDS-Page
Product Name

[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial (Pdk4), partial, Recombinant Protein

Popular Item
Also Known As

Recombinant Mouse [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial (Pdk4), partial

Product Synonym Names
[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial; EC=2.7.11.2; Pyruvate dehydrogenase kinase isoform 4
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS1216721 COA
Sequence Positions
138-368aa; Partial
Sequence Length
368
Sequence
ILEYKDTCTV DPVTNQNLQY FLDRFYMNRI STRMLMNQHI LIFSDSKTGN PSHIGSIDPN CDVVAVVQDA FECAKMLCDQ YYLTSPELNL TQVNGKFPGQ PIHIVYVPSH LHHMLFELFK NAMRATVEHQ ENRPSLTPVE ATVVLGKEDL TIKISDRGGG VPLRITDRLF SYTYSTAPTP VMDNSRNAPL AGFGYGLPIS RLYAKYFQGD LNLYSMSGYG TDAIIYLKAL S
3D Structure
ModBase 3D Structure for O70571
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>=90% (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Species
Mus musculus (Mouse)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of Pdk4 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
Pdk4 recombinant protein
Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid biosynthesis. Plays a role in the generation of reactive oxygen species. Protects detached epithelial cells against anoikis. Plays a role in cell proliferation via its role in regulating carbohydrate and fatty acid metabolism
Product Categories/Family for Pdk4 recombinant protein

Pdk4 recombinant protein SDS-Page image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
NCBI/Uniprot data below describe general gene information for Pdk4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
46,596 Da
NCBI Official Full Name
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial
NCBI Official Synonym Full Names
pyruvate dehydrogenase kinase, isoenzyme 4
NCBI Official Symbol
NCBI Official Synonym Symbols
AV005916
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NCBI Protein Information
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial; pyruvate dehydrogenase kinase 4; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial
UniProt Protein Name
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial
UniProt Synonym Protein Names
Pyruvate dehydrogenase kinase isoform 4
UniProt Gene Name
UniProt Entry Name
PDK4_MOUSE
UniProt Comments for Pdk4
PDHK4: an ubiquitously expressed, atypical protein kinase associated with the mitochondrial matrix. The PDHKs play crucial roles in switching metabolic flux from oxidative phosphorylation towards glycolysis. PDHK4 is abundant in heart, skeletal muscle, kidney, and pancreatic islets. Contains a HATPase_c catalytic domain, found in several ATP-binding proteins including protein histidine kinases (PHKs), PHDKs, DNA gyrase B, topoisomerases, heat shock proteins, and DNA mismatch repair proteins. PDHK regulates glucose oxidation through inhibitory phosphorylation of the E1 alpha subunit of the mitochondrial pyruvate dehydrogenase complex (PDHC) at any one of 3 inhibitory serine residues. Inhibitory sites 1, 2, and 3 correspond to S293, S300, and S232 in human PDHA1, respectively. Four PDHK isoenzymes have been described, each with different site specificity: all four phosphorylate sites 1 and 2 but at different rates; for site 1 PDHK2 >PDHK4 >PDHK1 >PDHK3; for site 2, PDHK3> PDHK4 > PDHK2 > PDHK1. Only PDHK1 phosphorylates site 3. PDHKs are recruited to the PDHC by binding to a lipoyl group covalently attached to the inner lipoyl domain of the E2 component. PDHA1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. Suppression of PDH by PDHK inhibits the conversion of pyruvate to acetyl-CoA, attenuates mitochondrial respiration, and may contribute to the increased lactate production observed in many tumors. The PDH pathway is repressed in a majority of non-small cell lung carcinomas. Inhibited by AZD7545, dichloroacetate (DCA), and radicicol. Radicicol inhibits kinase activity by binding directly to the ATP-binding pocket of PDHK, similar to HSP90 from the same ATPase/kinase superfamily.

Protein type: EC 2.7.11.2; Protein kinase, atypical; Kinase, protein; Mitochondrial; ATYPICAL group; PDHK family

Cellular Component: mitochondrion; mitochondrial inner membrane

Molecular Function: transferase activity; nucleotide binding; protein serine/threonine/tyrosine kinase activity; kinase activity; pyruvate dehydrogenase (acetyl-transferring) kinase activity; ATP binding; protein kinase activity

Biological Process: acetyl-CoA biosynthetic process from pyruvate; regulation of bone resorption; glucose metabolic process; regulation of acetyl-CoA biosynthetic process from pyruvate; cellular response to starvation; glucose homeostasis; protein amino acid phosphorylation; response to starvation; regulation of fatty acid biosynthetic process; carbohydrate metabolic process; regulation of fatty acid oxidation; insulin receptor signaling pathway; regulation of pH; phosphorylation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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