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PEBP1 elisa kit :: Human phosphatidylethanolamine binding protein 1 ELISA Kit

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Catalog # MBS902854
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Typical Testing Data/Standard Curve (for reference only)
Product Name

phosphatidylethanolamine binding protein 1 (PEBP1), ELISA Kit

Popular Item
Full Product Name

Human Phosphatidylethanolamine-binding protein 1, PEBP1 ELISA Kit

Product Synonym Names
Human Phosphatidylethanolamine-binding protein 1 (PEBP1) ELISA kit; HCNP; PBP; PEBP; RKIP; Raf kinase inhibitory protein; hippocampal cholinergic neurostimulating peptide; prostatic binding protein; phosphatidylethanolamine binding protein 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS902854 Testing Data
OMIM
604591
3D Structure
ModBase 3D Structure for P30086
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human PEBP1. No significant cross-reactivity or interference between human PEBP1 and analogues was observed.
Samples
Serum, plasma, cell lysates
Assay Type
Quantitative Sandwich
Detection Range
0.156 ng/ml -10 ng/ml
Sensitivity
0.039 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PEBP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for PEBP1purchase
MBS902854 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the phosphatidylethanolamine binding protein 1 (PEBP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PEBP1. The ELISA analytical biochemical technique of the MBS902854 kit is based on PEBP1 antibody-PEBP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PEBP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PEBP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PEBP1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PEBP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PEBP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PEBP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PEBP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of PEBP1 elisa kit
PEBP1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS902854. Click to request current manual
NCBI/Uniprot data below describe general gene information for PEBP1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
21,057 Da
NCBI Official Full Name
phosphatidylethanolamine-binding protein 1 preproprotein
NCBI Official Synonym Full Names
phosphatidylethanolamine binding protein 1
NCBI Official Symbol
PEBP1  [Similar Products]
NCBI Official Synonym Symbols
PBP; HCNP; PEBP; RKIP; HCNPpp; PEBP-1; HEL-210; HEL-S-34
  [Similar Products]
NCBI Protein Information
phosphatidylethanolamine-binding protein 1; neuropolypeptide h3; prostatic binding protein; prostatic-binding protein; raf kinase inhibitor protein; Raf kinase inhibitory protein; epididymis luminal protein 210; epididymis secretory protein Li 34; hippocampal cholinergic neurostimulating peptide
UniProt Protein Name
Phosphatidylethanolamine-binding protein 1
UniProt Synonym Protein Names
HCNPpp; Neuropolypeptide h3; Prostatic-binding protein; Raf kinase inhibitor protein; RKIP
UniProt Gene Name
PEBP1  [Similar Products]
UniProt Synonym Gene Names
PBP; PEBP; PEBP-1; RKIP; HCNP  [Similar Products]
UniProt Entry Name
PEBP1_HUMAN
NCBI Summary for PEBP1
This gene encodes a member of the phosphatidylethanolamine-binding family of proteins and has been shown to modulate multiple signaling pathways, including the MAP kinase (MAPK), NF-kappa B, and glycogen synthase kinase-3 (GSK-3) signaling pathways. The encoded protein can be further processed to form a smaller cleavage product, hippocampal cholinergic neurostimulating peptide (HCNP), which may be involved in neural development. This gene has been implicated in numerous human cancers and may act as a metastasis suppressor gene. Multiple pseudogenes of this gene have been identified in the genome. [provided by RefSeq, Jul 2015]
UniProt Comments for PEBP1
RKIP: a metastasis suppressor that regulates extracellular matrix remodeling and tumor survival. Binds to and inhibits Raf-1. Phosphorylation by classic and atypical but not novel PKC isoforms dissociates this complex, relieving inhibition of the Raf/MAP kinase signaling cascade. Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation. Cleaved into hippocampal cholinergic neurostimulating peptide (HCMP) that appears to be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Expressed in brain. Increased expression in aged senescence-accelerated mice.

Protein type: Lipid-binding; Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 12q24.23

Cellular Component: cytoplasm; nucleus

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; enzyme binding; phosphatidylethanolamine binding; protein kinase binding; ATP binding
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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