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PECAM-1/CD31 elisa kit :: Rat Platelet endothelial cell adhesion molecule-1 ELISA Kit

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Catalog # MBS724018
Unit / Price
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  48-Strip-Wells-(Competitive)  /  $440 +1 FREE 8GB USB
  48-Strip-Wells-(Sandwich)  /  $440 +1 FREE 8GB USB
  96-Strip-Wells-(Competitive)  /  $640 +1 FREE 8GB USB
  96-Strip-Wells-(Sandwich)  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells-(Competitive)  /  $2,895 +3 FREE 8GB USB
  5x96-Strip-Wells-(Sandwich)  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells-(Competitive)  /  $5,415 +6 FREE 8GB USB
  10x96-Strip-Wells-(Sandwich)  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31), ELISA Kit

Full Product Name

Rat Platelet endothelial cell adhesion molecule-1 ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
173445
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive or Sandwich
Detection Range
100-2500 pg/mL
Sensitivity
1.0pg/ml
Intended Uses
This CD31 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CD31. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PECAM-1/CD31 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for PECAM-1/CD31purchase
MBS724018 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PECAM-1/CD31. The ELISA analytical biochemical technique of the MBS724018 kit is based on PECAM-1/CD31 antibody-PECAM-1/CD31 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PECAM-1/CD31 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PECAM-1/CD31. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PECAM-1/CD31 elisa kit
Principle of the Assay: CD31 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CD31. Standards or samples are then added to the microtiter plate wells and CD31 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CD31 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for CD31 are added to each well to "sandwich" the CD31 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CD31 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD31 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for PECAM-1/CD31 elisa kit

Typical Testing Data/Standard Curve (for reference only) of PECAM-1/CD31 elisa kit
PECAM-1/CD31 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS724018. Click to request current manual
NCBI/Uniprot data below describe general gene information for PECAM-1/CD31. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
81,378 Da
NCBI Official Full Name
platelet endothelial cell adhesion molecule
NCBI Official Synonym Full Names
platelet/endothelial cell adhesion molecule 1
NCBI Official Symbol
PECAM1  [Similar Products]
NCBI Official Synonym Symbols
CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
  [Similar Products]
NCBI Protein Information
platelet endothelial cell adhesion molecule; CD31 antigen
UniProt Protein Name
Platelet endothelial cell adhesion molecule
UniProt Synonym Protein Names
EndoCAM; GPIIA'; PECA1; CD_antigen: CD31
UniProt Gene Name
PECAM1  [Similar Products]
UniProt Synonym Gene Names
PECAM-1  [Similar Products]
UniProt Entry Name
PECA1_HUMAN
NCBI Summary for PECAM-1/CD31
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]
UniProt Comments for PECAM-1/CD31
PECAM-1: a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Plays a role in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, cell migration, negative regulation of immune cell signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. Undergoes alternative splicing, generating multiple isoforms in vascular beds of various tissues.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: extracellular space; platelet alpha granule membrane; plasma membrane; integral to membrane; cell junction

Molecular Function: protein binding

Biological Process: platelet activation; extracellular matrix organization and biogenesis; platelet degranulation; diapedesis; cell recognition; blood coagulation; cell adhesion; signal transduction; leukocyte migration; phagocytosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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