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PEPD elisa kit :: Human Xaa-Pro dipeptidase ELISA Kit

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Catalog # MBS2883088
Unit / Price
  48-Strip-Wells  /  $400 +1 FREE 8GB USB
  96-Strip-Wells  /  $530 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,305 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $4,250 +5 FREE 8GB USB
 Typical Testing Data/Standard Curve (for reference only)
Product Name

Xaa-Pro dipeptidase (PEPD), ELISA Kit

Popular Item
Also Known As

Human Xaa-Pro dipeptidase ELISA Kit

Product Synonym Names
Xaa-Pro dipeptidase; X-Pro dipeptidase; Imidodipeptidase; Peptidase D; Proline dipeptidase; Prolidase; PEPD; PRD;
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2883088 Testing Data
Sequence Length
3D Structure
ModBase 3D Structure for P12955
Species Reactivity
This assay recognizes recombinant and natural human PEPD. No significant cross-reactivity or interference was observed.
Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids
Detection Range
0.78 ng/mL -50.0 ng/mL.
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PEPD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPEPDpurchase
MBS2883088 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Xaa-Pro dipeptidase (PEPD) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PEPD. The ELISA analytical biochemical technique of the MBS2883088 kit is based on PEPD antibody-PEPD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PEPD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PEPD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PEPD elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human xaa-pro dipeptidase,PEPD concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PEPD. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PEPD and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PEPD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PEPD in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of PEPD elisa kit
PEPD elisa kit  Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2883088. Click to request current manual
NCBI/Uniprot data below describe general gene information for PEPD. It may not necessarily be applicable to this product.
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
47,261 Da
NCBI Official Full Name
xaa-Pro dipeptidase isoform 1
NCBI Official Synonym Full Names
peptidase D
NCBI Official Symbol
NCBI Official Synonym Symbols
  [Similar Products]
NCBI Protein Information
xaa-Pro dipeptidase
UniProt Protein Name
Xaa-Pro dipeptidase
UniProt Synonym Protein Names
Imidodipeptidase; Peptidase D; Proline dipeptidase; Prolidase
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
PRD; X-Pro dipeptidase; Prolidase  [Similar Products]
UniProt Entry Name
NCBI Summary for PEPD
This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]
UniProt Comments for PEPD
peptidase D: Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because the high level of iminoacids in collagen. Defects in PEPD are a cause of prolidase deficiency (PD). Prolidase deficiency is an autosomal recessive disorder associated with iminodipeptiduria. The clinical phenotype includes skin ulcers, mental retardation, recurrent infections, and a characteristic facies. These features, however are incompletely penetrant and highly variable in both age of onset and severity. There is a tight linkage between the polymorphisms of prolidase and the myotonic dystrophy trait. Belongs to the peptidase M24B family. Eukaryotic-type prolidase subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC

Chromosomal Location of Human Ortholog: 19q13.11

Molecular Function: aminopeptidase activity; dipeptidase activity; manganese ion binding; metallocarboxypeptidase activity; protein binding

Biological Process: amino acid metabolic process; collagen catabolic process; proteolysis

Disease: Prolidase Deficiency
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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