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PERK elisa kit :: Human Phospho-Exiracellular Signal-Regulated Kinase ELISA Kit

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Catalog # MBS014568
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  48-Strip-Wells  /  $470 +1 FREE 8GB USB
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PERK elisa kit
Product Name

Phospho-Exiracellular Signal-Regulated Kinase (PERK), ELISA Kit

Popular Item
Full Product Name

Human Phospho-Exiracellular Signal-Regulated Kinase ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
606108
3D Structure
ModBase 3D Structure for P35813
Species Reactivity
Specificity
No significant cross-reactivity or interference between Human PERK and analogues was observed.
Samples
Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
Assay Type
Sandwich
Detection Range
125 pg/ml – 4000 pg/ml.
Sensitivity
10 pg/ml.
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PERK concentrations in Human serum, plasma and other body fluids. Using Purified Human PERK antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PERK and PERK antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PERK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PERK elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for PERKpurchase
MBS014568 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phospho-Exiracellular Signal-Regulated Kinase (PERK) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PERK. The ELISA analytical biochemical technique of the MBS014568 kit is based on PERK antibody-PERK antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PERK antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PERK. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PERK elisa kit
Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PERK concentrations in Human serum, plasma and other body fluids. Using Purified Human PERK antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PERK and PERK antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PERK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS014568. Click to request current manual
NCBI/Uniprot data below describe general gene information for PERK. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
42,448 Da
NCBI Official Full Name
protein phosphatase 1A isoform 3
NCBI Official Synonym Full Names
protein phosphatase, Mg2+/Mn2+ dependent, 1A
NCBI Official Symbol
PPM1A  [Similar Products]
NCBI Official Synonym Symbols
PP2CA; PP2Calpha; PP2C-ALPHA
  [Similar Products]
NCBI Protein Information
protein phosphatase 1A; protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform
UniProt Protein Name
Protein phosphatase 1A
UniProt Synonym Protein Names
Protein phosphatase 2C isoform alpha; PP2C-alpha; Protein phosphatase IA
UniProt Gene Name
PPM1A  [Similar Products]
UniProt Synonym Gene Names
PPPM1A; PP2C-alpha  [Similar Products]
UniProt Entry Name
PPM1A_HUMAN
NCBI Summary for PERK
The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Comments for PERK
PPPM1A: a Ser/Thr protein phosphatase of the PP2C family. Inhibits the activation of p38 and JNK kinase cascades induced by environmental stresses. Can also dephosphorylate cyclin-dependent kinases and may play a role in cell cycle control. Overexpression of this phosphatase activates the expression of p53, leading to G2/M cell cycle arrest and apoptosis.

Protein type: Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q23.1

Cellular Component: nucleoplasm; neuron projection; membrane; voltage-gated calcium channel complex; nucleus; cytosol

Molecular Function: protein C-terminus binding; signal transducer activity; protein binding; calmodulin-dependent protein phosphatase activity; manganese ion binding; magnesium ion binding; protein serine/threonine phosphatase activity

Biological Process: N-terminal protein myristoylation; transcription initiation from RNA polymerase II promoter; Wnt receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; negative regulation of NF-kappaB import into nucleus; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of transcription from RNA polymerase II promoter; protein amino acid dephosphorylation; dephosphorylation; transforming growth factor beta receptor signaling pathway; insulin receptor signaling pathway; gene expression; negative regulation of transforming growth factor beta receptor signaling pathway; cell cycle arrest; positive regulation of Wnt receptor signaling pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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