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PEX recombinant protein :: C-terminal hemopexin-like domain of MMP-2 Recombinant Protein

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Catalog # MBS142915
Unit / Price
  0.002 mg  /  $140 +1 FREE 8GB USB
  0.01 mg  /  $205 +1 FREE 8GB USB
  1 mg  /  $3,975 +4 FREE 8GB USB
PEX recombinant protein
Product Name

C-terminal hemopexin-like domain of MMP-2, Recombinant Protein

Popular Item
Also Known As

Recombinant Human C-terminal hemopexin-like domain of MMP-2 (445-635 a.a.)

Product Synonym Names
PEX Human; C-terminal hemopexin-like domain of MMP-2 Human Recombinant; C-terminal hemopexin-like domain of MMP-2; PEX
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
749
Sequence
MGLEHSQDPG ALMAPIYTYT KNFRLSQDDI KGIQELYGAS PDIDLGTGPT PTLGPVTPEI CKQDIVFDGI AQIRGEIFFF KDRFIWRTVT PRDKPMGPLL VATFWPELPE KIDAVYEAPQ EEKAVFFAGN EYWIYSASTL ERGYPKPLTS LGLPPDVQRV DAAFNWSKNK KTYIFAGDKF WRYNEVKKKM DPGFPKLIAD AWNAIPDNLD AVVDLQGGGH SYFFKGAYYL KLENQSLKSV KFGSIKSDWL GC
OMIM
300550
Host
E Coli
Purity/Purification
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Form/Format
The protein was lyophilized with 2mM Tris pH-7.4.
Sterile Filtered White lyophilized (freeze-dried) powder.
Solubility
It is recommended to reconstitute the PEX in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Activity
The bioactivity was measured by HMEC cell line, PEX can inhibit the transmembrane activity of HMEC under the stimulation of VEGF.
Preparation and Storage
Lyophilized PEX although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution PEX should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Other Notes
Small volumes of PEX recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PEX recombinant protein
Description: PEX Human Recombinant is expressed as inclusion bodies in E Coli having a molecular mass of 28,453 Dalton and subsequently refolded in vitro to get biological activity.

Introduction: Recombinant Human PEX (C-terminal hemopexin-like domain of MMP-2) is a noncatalytic Metalloproteinase Fragment with Integrin Binding Activity and can inhibit cell associated collagenolytic activity both in vitro and in vivo. Moreover, PEX can block angiogenesis and tumor growth in vivo, providing a potentially novel therapeutic approach for diseases associated with neovascularization. The appearance of PEX at sites of neovascularization may not only control normal angiogenesis, but when administered in sufficient quantities, may provide a naturally-occurring therapeutic inhibitor of diseases associated with angiogenesis. PEX mediates interaction with inhibitors and the cell surface, and is vital for activation. PEX is composed of 4 sub-domains arranged as a 4-bladed propeller. PEX, which interferes with the cell membrane activation of MMP-2, reduced Rac1- promoted cell invasiveness as observed by collagen invasion assay. It has also been described that PEX prevents binding of MMP-2 to the integrin avb3.
NCBI/Uniprot data below describe general gene information for PEX. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
86,474 Da
NCBI Official Full Name
phosphate-regulating neutral endopeptidase isoform 1
NCBI Official Synonym Full Names
phosphate regulating endopeptidase homolog, X-linked
NCBI Official Symbol
NCBI Official Synonym Symbols
HYP; PEX; XLH; HPDR; HYP1; LXHR; HPDR1
  [Similar Products]
NCBI Protein Information
phosphate-regulating neutral endopeptidase; X-linked hypophosphatemia protein; metalloendopeptidase homolog PEX; phosphate regulating gene with homologies to endopeptidases on the X chromosome (hypophosphatemia, vitamin D resistant rickets); vitamin D-resistant hypophosphatemic rickets protein
UniProt Protein Name
Phosphate-regulating neutral endopeptidase
UniProt Synonym Protein Names
Metalloendopeptidase homolog PEX; Vitamin D-resistant hypophosphatemic rickets protein; X-linked hypophosphatemia protein; HYP
UniProt Gene Name
UniProt Synonym Gene Names
PEX; HYP  [Similar Products]
UniProt Entry Name
PHEX_HUMAN
NCBI Summary for PEX
The protein encoded by this gene is a transmembrane endopeptidase that belongs to the type II integral membrane zinc-dependent endopeptidase family. The protein is thought to be involved in bone and dentin mineralization and renal phosphate reabsorption. Mutations in this gene cause X-linked hypophosphatemic rickets. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
UniProt Comments for PEX
PHEX: Probably involved in bone and dentin mineralization and renal phosphate reabsorption. Defects in PHEX are a cause of hypophosphatemic rickets, X-linked dominant (XLHR). XLHR is an X-linked dominant disorder characterized by impaired phosphate uptake in the kidney, which is likely to be caused by abnormal regulation of sodium phosphate cotransport in the proximal tubules. Clinical manifestations include skeletal deformities, growth failure, craniosynostosis, paravertebral calcifications, pseudofractures in lower extremities, and muscular hypotonia with onset in early childhood. X-linked hypophosphatemic rickets is the most common form of hypophosphatemia with an incidence of 1 in 20000. Belongs to the peptidase M13 family.

Protein type: EC 3.4.24.-; Protease; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.2-p22.1

Cellular Component: Golgi apparatus; integral to plasma membrane; endoplasmic reticulum; perinuclear region of cytoplasm; plasma membrane

Molecular Function: zinc ion binding; metalloendopeptidase activity; aminopeptidase activity

Biological Process: cell-cell signaling; protein modification process; organophosphate metabolic process; proteolysis; skeletal development; bone mineralization

Disease: Hypophosphatemic Rickets, X-linked Dominant
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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