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PF4V1 elisa kit :: Human PF4V1 ELISA Kit

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Catalog # MBS2507520
Unit / Price
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  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

PF4V1, ELISA Kit

Popular Item
Full Product Name

Human PF4V1 (Platelet Factor 4 Variant 1) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2507520 COA
OMIM
173461
3D Structure
ModBase 3D Structure for P10720
Species Reactivity
Specificity
This kit recognizes natural and recombinant Human PF4V1. No significant cross-reactivity or interference between Human PF4V1 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
1.563-100ng/mL
Sensitivity
Min: 0.938ng/mL; Max: 100ng/mL
Intended Uses
This ELISA kit applies to the in vitro quantitative determination of Human PF4V1 concentrations in serum, plasma and other biological fluids.
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PF4V1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPF4V1purchase
MBS2507520 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PF4V1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PF4V1. The ELISA analytical biochemical technique of the MBS2507520 kit is based on PF4V1 antibody-PF4V1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PF4V1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PF4V1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PF4V1 elisa kit
Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PF4V1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PF4V1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PF4V1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PF4V1. You can calculate the concentration of PF4V1 in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of PF4V1 elisa kit
PF4V1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2507520. Click to request current manual
NCBI/Uniprot data below describe general gene information for PF4V1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
11,553 Da
NCBI Official Full Name
platelet factor 4 variant
NCBI Official Synonym Full Names
platelet factor 4 variant 1
NCBI Official Symbol
PF4V1  [Similar Products]
NCBI Official Synonym Symbols
PF4A; CXCL4L1; CXCL4V1; PF4-ALT; SCYB4V1
  [Similar Products]
NCBI Protein Information
platelet factor 4 variant; C-X-C motif chemokine 4; PF4alt; PF4var1; platelet factor 4, variant 1 (PF4-like)
UniProt Protein Name
Platelet factor 4 variant
UniProt Synonym Protein Names
C-X-C motif chemokine 4 variant; CXCL4L1; PF4alt; PF4var1Cleaved into the following 3 chains:Platelet factor 4 variant(4-74); Platelet factor 4 variant(5-74); Platelet factor 4 variant(6-74)
Protein Family
UniProt Gene Name
PF4V1  [Similar Products]
UniProt Synonym Gene Names
CXCL4V1; SCYB4V1  [Similar Products]
UniProt Entry Name
PF4V_HUMAN
UniProt Comments for PF4V1
PF4V1: Inhibitor of angiogenesis. Inhibitor of endothelial cell chemotaxis (in vitro). Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Chemokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q12-q21

Cellular Component: extracellular space

Molecular Function: heparin binding; chemokine activity; CXCR chemokine receptor binding

Biological Process: G-protein coupled receptor protein signaling pathway; response to lipopolysaccharide; immune response; positive regulation of leukocyte chemotaxis; inflammatory response; regulation of cell proliferation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withPF4V1 elisa kit
 Reference Product  PubMed Publications
 CXCR3 elisa kit  >4 publications with PF4V1 and CXCR3
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