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PFKFB1 elisa kit :: Chicken 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 1 (PFKFB1) ELISA Kit

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Catalog # MBS7201431
Unit / Price
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  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
PFKFB1 elisa kit
Product Name

6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 1 (PFKFB1), ELISA Kit

Full Product Name

Chicken 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 1 (PFKFB1) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
311790
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PFKFB1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for PFKFB1purchase
MBS7201431 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 1 (PFKFB1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PFKFB1. The ELISA analytical biochemical technique of the MBS7201431 kit is based on PFKFB1 antibody-PFKFB1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PFKFB1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PFKFB1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Product Categories/Family for PFKFB1 elisa kit
NCBI/Uniprot data below describe general gene information for PFKFB1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
47,426 Da
NCBI Official Full Name
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
NCBI Official Synonym Full Names
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
NCBI Official Symbol
PFKFB1  [Similar Products]
NCBI Official Synonym Symbols
F6PK; HL2K; PFRX
  [Similar Products]
NCBI Protein Information
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1; PFK/FBPase 1; 6PF-2-K/Fru-2,6-P2ase 1; 6PF-2-K/Fru-2,6-P2ASE liver isozyme; fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase
UniProt Protein Name
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1
UniProt Synonym Protein Names
6PF-2-K/Fru-2,6-P2ase liver isozymeIncluding the following 2 domains:6-phosphofructo-2-kinase (EC:2.7.1.105); Fructose-2,6-bisphosphatase (EC:3.1.3.46)
UniProt Gene Name
PFKFB1  [Similar Products]
UniProt Synonym Gene Names
F6PK; PFRX; 6PF-2-K/Fru-2,6-P2ase 1; PFK/FBPase 1  [Similar Products]
UniProt Entry Name
F261_HUMAN
NCBI Summary for PFKFB1
This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012]
UniProt Comments for PFKFB1
PFKFB1: Synthesis and degradation of fructose 2,6-bisphosphate. Homodimer. Liver. Phosphorylation results in inhibition of the kinase activity.

Protein type: Kinase, other; Motility/polarity/chemotaxis; Carbohydrate Metabolism - fructose and mannose; EC 3.1.3.46; EC 2.7.1.105; Phosphatase (non-protein)

Chromosomal Location of Human Ortholog: Xp11.21

Cellular Component: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 complex; cytosol

Molecular Function: identical protein binding; protein binding; fructose-2,6-bisphosphate 2-phosphatase activity; 6-phosphofructo-2-kinase activity; kinase binding; ATP binding

Biological Process: organ regeneration; glycolysis; response to cAMP; response to glucagon stimulus; response to glucocorticoid stimulus; glucose metabolic process; pathogenesis; fructose 2,6-bisphosphate metabolic process; gluconeogenesis; response to insulin stimulus; response to starvation; dephosphorylation; carbohydrate metabolic process; carbohydrate phosphorylation; energy reserve metabolic process; fructose metabolic process; positive regulation of glucokinase activity
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated with PFKFB1 elisa kit
 Reference Product  PubMed Publications
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Organs/Tissues associated with PFKFB1 elisa kit
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