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PFKFB2 elisa kit :: Monkey 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 2 (PFKFB2) ELISA Kit

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Catalog # MBS7225695
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
PFKFB2 elisa kit
Product Name

6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 2 (PFKFB2), ELISA Kit

Full Product Name

Monkey 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 2 (PFKFB2) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
171835
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PFKFB2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPFKFB2purchase
MBS7225695 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 2 (PFKFB2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PFKFB2. The ELISA analytical biochemical technique of the MBS7225695 kit is based on PFKFB2 antibody-PFKFB2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PFKFB2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PFKFB2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Product Categories/Family for PFKFB2 elisa kit
NCBI/Uniprot data below describe general gene information for PFKFB2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
54,406 Da
NCBI Official Full Name
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
NCBI Official Synonym Full Names
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
NCBI Official Symbol
PFKFB2  [Similar Products]
NCBI Official Synonym Symbols
PFK-2/FBPase-2
  [Similar Products]
NCBI Protein Information
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2; PFK/FBPase 2; PFKFB, cardiac; 6PF-2-K/Fru-2,6-P2ase 2; 6PF-2-K/Fru-2,6-P2ASE heart-type isozyme; fructose-2,6-bisphosphatase, cardiac isozyme
UniProt Protein Name
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2
UniProt Synonym Protein Names
6PF-2-K/Fru-2,6-P2ase heart-type isozymeIncluding the following 2 domains:6-phosphofructo-2-kinase (EC:2.7.1.105); Fructose-2,6-bisphosphatase (EC:3.1.3.46)
UniProt Gene Name
PFKFB2  [Similar Products]
UniProt Synonym Gene Names
6PF-2-K/Fru-2,6-P2ase 2; PFK/FBPase 2  [Similar Products]
UniProt Entry Name
F262_HUMAN
NCBI Summary for PFKFB2
The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for PFKFB2
PFKFB2: a bifunctional heterodimeric enzyme involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. Regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. Two splice-variant isoforms have been described.

Protein type: Phosphatase (non-protein); Carbohydrate Metabolism - fructose and mannose; EC 2.7.1.105; Kinase, other; EC 3.1.3.46; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q31

Cellular Component: cytosol

Molecular Function: protein binding; fructose-2,6-bisphosphate 2-phosphatase activity; 6-phosphofructo-2-kinase activity; protein kinase binding; ATP binding

Biological Process: glycolysis; positive regulation of insulin secretion; glucose metabolic process; pathogenesis; fructose 2,6-bisphosphate metabolic process; glucose catabolic process; dephosphorylation; lactate metabolic process; carbohydrate phosphorylation; carbohydrate metabolic process; response to glucose stimulus; positive regulation of glucokinase activity; fructose metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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