• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

PFKL elisa kit :: Human 6-phosphofructokinase, liver type ELISA Kit

Scan QR to view Datasheet
Catalog # MBS2887722
Unit / Price
Scan QR to view Datasheet
  48-Strip-Wells  /  $400 +1 FREE 8GB USB
  96-Strip-Wells  /  $530 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,305 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $4,250 +5 FREE 8GB USB
PFKL elisa kit
Product Name

6-phosphofructokinase, liver type (PFKL), ELISA Kit

Full Product Name

Human 6-phosphofructokinase, liver type ELISA Kit

Product Synonym Names
6-phosphofructokinase, liver type; Phosphofructo-1-kinase isozyme B; PFK-B; Phosphofructokinase 1; Phosphohexokinase; PFKL; 2.7.1.11
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
171860
3D Structure
ModBase 3D Structure for P17858
Species Reactivity
Assay Type
Sandwich
Detection Range
1.56-100 ng/mL
Sensitivity
0.787 ng/mL
Intra-Assay CV
<=6.9%
Inter-Assay CV
<=9.7%
Recovery
95%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PFKL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for PFKLpurchase
MBS2887722 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 6-phosphofructokinase, liver type (PFKL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PFKL. The ELISA analytical biochemical technique of the MBS2887722 kit is based on PFKL antibody-PFKL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PFKL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PFKL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
NCBI/Uniprot data below describe general gene information for PFKL. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
90,203 Da
NCBI Official Full Name
ATP-dependent 6-phosphofructokinase, liver type isoform a
NCBI Official Synonym Full Names
phosphofructokinase, liver type
NCBI Official Symbol
NCBI Official Synonym Symbols
PFK-B; PFK-L; ATP-PFK
  [Similar Products]
NCBI Protein Information
ATP-dependent 6-phosphofructokinase, liver type
UniProt Protein Name
ATP-dependent 6-phosphofructokinase, liver type
UniProt Synonym Protein Names
6-phosphofructokinase type B; Phosphofructo-1-kinase isozyme B; PFK-B; Phosphohexokinase
UniProt Gene Name
UniProt Synonym Gene Names
ATP-PFK; PFK-L  [Similar Products]
UniProt Entry Name
PFKAL_HUMAN
NCBI Summary for PFKL
This gene encodes the liver (L) subunit of an enzyme that catalyzes the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate, which is a key step in glucose metabolism (glycolysis). This enzyme is a tetramer that may be composed of different subunits encoded by distinct genes in different tissues. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
UniProt Comments for PFKL
PFKL: phosphofructokinase, liver type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts. Two alternatively spliced isoforms have been described.

Protein type: Kinase, other; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pentose phosphate pathway; EC 2.7.1.11; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - galactose

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: 6-phosphofructokinase complex; cytosol; membrane

Molecular Function: 6-phosphofructokinase activity; ATP binding; identical protein binding; kinase binding; metal ion binding; protein binding

Biological Process: carbohydrate metabolic process; fructose 1,6-bisphosphate metabolic process; fructose 6-phosphate metabolic process; glucose metabolic process; glycolysis; negative regulation of insulin secretion; protein homotetramerization; protein oligomerization; response to glucose stimulus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000