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PFKP elisa kit :: Hamster Phosphofructokinase- Platelet ELISA Kit

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Catalog # MBS006175
Unit / Price
  48-Strip-Wells  /  $470 +1 FREE 8GB USB
  96-Strip-Wells  /  $680 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
PFKP elisa kit
Product Name

Phosphofructokinase- Platelet (PFKP), ELISA Kit

Also Known As

Hamster Phosphofructokinase- Platelet ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Chromosome Location
Chromosome: 10; NC_000010.10 (3109712..3178997). Location: 10p15.3-p15.2
OMIM
171840
3D Structure
ModBase 3D Structure for Q01813
Species Reactivity
Assay Type
Sandwich
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PFKP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPFKPpurchase
MBS006175 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phosphofructokinase- Platelet (PFKP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PFKP. The ELISA analytical biochemical technique of the MBS006175 kit is based on PFKP antibody-PFKP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PFKP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PFKP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
NCBI/Uniprot data below describe general gene information for PFKP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
85,596 Da
NCBI Official Full Name
PFKP protein
NCBI Official Synonym Full Names
phosphofructokinase, platelet
NCBI Official Symbol
NCBI Official Synonym Symbols
PFKF; PFK-C
  [Similar Products]
NCBI Protein Information
6-phosphofructokinase type C; phosphohexokinase; phosphofructokinase 1; phosphofructo-1-kinase isozyme C; Phosphofructokinase, platelet type; 6-phosphofructokinase, platelet type
UniProt Protein Name
6-phosphofructokinase type C
UniProt Synonym Protein Names
6-phosphofructokinase, platelet type; Phosphofructo-1-kinase isozyme C; PFK-C; Phosphofructokinase 1; Phosphohexokinase
UniProt Gene Name
UniProt Synonym Gene Names
PFKF; PFK-C  [Similar Products]
UniProt Entry Name
K6PP_HUMAN
NCBI Summary for PFKP
The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM; MIM 610681) and liver (PFKL; MIM 171860) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora (1981) [PubMed 6451249] determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.[supplied by OMIM, Mar 2008]
UniProt Comments for PFKP
PFKP: phosphofructokinase, platelet type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - galactose; Kinase, other; EC 2.7.1.11; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - pentose phosphate pathway

Chromosomal Location of Human Ortholog: 10p15.3-p15.2

Cellular Component: membrane; cytosol; nucleus

Molecular Function: metal ion binding; protein complex binding; ATP binding; 6-phosphofructokinase activity

Biological Process: glycolysis; carbohydrate metabolic process; carbohydrate phosphorylation; glucose metabolic process; pathogenesis; fructose 6-phosphate metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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