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PGM5 elisa kit :: Human Phosphoglucomutase-like protein 5 ELISA Kit

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Catalog # MBS283563
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  48-Strip-Wells  /  $405 +1 FREE 8GB USB
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PGM5 elisa kit
Product Name

Phosphoglucomutase-like protein 5 (PGM5), ELISA Kit

Full Product Name

Human Phosphoglucomutase-like protein 5 (PGM5) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
AK297108 mRNA
3D Structure
ModBase 3D Structure for Q15124
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human PGM5. No significant cross-reactivity or interference between Human PGM5 and analogues was observed.
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PGM5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for PGM5purchase
MBS283563 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phosphoglucomutase-like protein 5 (PGM5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PGM5. The ELISA analytical biochemical technique of the MBS283563 kit is based on PGM5 antibody-PGM5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PGM5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PGM5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PGM5 elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PGM5 in samples. An antibody specific for PGM5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPGM5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGM5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGM5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for PGM5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
41,790 Da
NCBI Official Full Name
phosphoglucomutase-like protein 5
NCBI Official Synonym Full Names
phosphoglucomutase 5
NCBI Official Symbol
NCBI Official Synonym Symbols
PGMRP
  [Similar Products]
NCBI Protein Information
phosphoglucomutase-like protein 5
UniProt Protein Name
Phosphoglucomutase-like protein 5
UniProt Synonym Protein Names
Aciculin; Phosphoglucomutase-related protein; PGM-RP
UniProt Gene Name
UniProt Synonym Gene Names
PGMRP; PGM-RP  [Similar Products]
NCBI Summary for PGM5
Phosphoglucomutases (EC 5.2.2.2.), such as PGM5, are phosphotransferases involved in interconversion of glucose-1-phosphate and glucose-6-phosphate. PGM activity is essential in formation of carbohydrates from glucose-6-phosphate and in formation of glucose-6-phosphate from galactose and glycogen (Edwards et al., 1995 [PubMed 8586438]).[supplied by OMIM, Mar 2008]
UniProt Comments for PGM5
PGM5: Component of adherens-type cell-cell and cell-matrix junctions. Lacks phosphoglucomutase activity. Belongs to the phosphohexose mutase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 9q21.11

Cellular Component: cell-cell adherens junction; costamere; cytoplasmic side of plasma membrane; cytosol; dystrophin-associated glycoprotein complex; focal adhesion; intercalated disc; sarcolemma; spot adherens junction; stress fiber; Z disc

Molecular Function: magnesium ion binding; phosphoglucomutase activity; structural molecule activity

Biological Process: cell adhesion; galactose catabolic process; glucose metabolic process; glycogen biosynthetic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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