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PHB elisa kit :: Human prohibitin ELISA Kit

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Catalog # MBS920981
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  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
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Typical Testing Data/Standard Curve (for reference only)
Product Name

prohibitin (PHB), ELISA Kit

Popular Item
Full Product Name

Human Prohibitin, PHB ELISA Kit

Product Synonym Names
Human Prohibitin (PHB) ELISA kit; PHB1; ; prohibitin
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS920981 Testing Data
OMIM
176705
3D Structure
ModBase 3D Structure for P35232
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human PHB. No significant cross-reactivity or interference between human PHB and analogues was observed.
Samples
serum, plasma, tissue homogenates, cell lysates
Assay Type
Quantitative Sandwich
Detection Range
23.5 pg/ml -1500 pg/ml
Sensitivity
<5.8 pg/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PHB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for PHBpurchase
MBS920981 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the prohibitin (PHB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PHB. The ELISA analytical biochemical technique of the MBS920981 kit is based on PHB antibody-PHB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PHB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PHB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PHB elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PHB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PHB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PHB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of PHB elisa kit
PHB elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS920981. Click to request current manual
NCBI/Uniprot data below describe general gene information for PHB. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
29,804 Da
NCBI Official Full Name
prohibitin isoform 1
NCBI Official Synonym Full Names
prohibitin
NCBI Official Symbol
NCBI Official Synonym Symbols
PHB1; HEL-215; HEL-S-54e
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NCBI Protein Information
prohibitin; epididymis luminal protein 215; epididymis secretory sperm binding protein Li 54e
UniProt Protein Name
Prohibitin
Protein Family
UniProt Gene Name
UniProt Entry Name
PHB_HUMAN
NCBI Summary for PHB
This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
UniProt Comments for PHB
PHB: a pleiotropic membrane protein that suppresses cell proliferation, apoptosis and senescence. It plays a role both in maintaining mitochondrial integrity and in cell cycle regulation. Expression increases approximately 3-fold upon entry into G1 phase compared with other phases of the cell cycle. It helps prevent ROS-induced senescence and its expression decreases heterogeneously during cellular aging. Helps maintain the angiogenic capacity of endothelial cells. Up-regulated during activation of primary human T cells via CD3/CD28 pathways. Present in multiple cellular compartments. Large assemblies of PHB and PHB2 localize in the inner membrane of mitochondria. Acts as a mitochondrial chaperone protein, regulates mitochondrial morphogenesis, and helps regulate the organization of mitochondrial DNA. Reported to target lipid rafts. In the nucleus, it has been reported to interact with various transcription factors, modulating their activity. A potential tumor suppressor that functions as a potent transcriptional corepressor for estrogen receptor alpha. It is downregulated by androgens, and represses androgen receptor activity. Co-localizes with Rb in the nucleus and recruits N-CoR and HDAC1 for transcriptional repression. In vivo promoter occupancy studies have suggested that the PHB gene is a direct target of c-Myc. May bind to and enhance the transcriptional activity of p53. Both Phb and Phb2 are present in the circulation and can be internalized by cultured cells. Overexpressed in endometrioid ovarian adenocarcinoma and papillary serous ovarian carcinoma. Mutated in sporadic breast cancer.

Protein type: Chaperone; Apoptosis; Oncoprotein; Cell cycle regulation; Nuclear receptor co-regulator; Mitochondrial; Transcription, coactivator/corepressor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: nucleoplasm; mitochondrion; membrane; integral to plasma membrane; cytoplasm; mitochondrial inner membrane; nucleus

Molecular Function: protein binding; enzyme binding; histone deacetylase binding

Biological Process: transcription from RNA polymerase II promoter; osteoblast differentiation; regulation of apoptosis; negative regulation of cell proliferation; progesterone receptor signaling pathway; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; histone deacetylation; negative regulation of cell growth; negative regulation of transcription from RNA polymerase II promoter; signal transduction; negative regulation of transcription, DNA-dependent

Disease: Breast Cancer
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with PHB elisa kit
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