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PIK3R2 sirna

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Catalog # MBS8230642
Unit / Price
  15 nmol  /  $365 +1 FREE 8GB USB
  30 nmol  /  $515 +1 FREE 8GB USB
PIK3R2 sirna
Product Name

PIK3R2, siRNA

Popular Item
Also Known As

PIK3R2 siRNA (Human)

Product Synonym Names
Phosphatidylinositol 3-kinase regulatory subunit beta; PI3-kinase regulatory subunit beta; PI3K regulatory subunit beta; PtdIns-3-kinase regulatory subunit beta; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; PI3-kinase subunit p85-beta; PtdIns-3-kinase regulatory subunit p85-beta; Pt
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
728
OMIM
603157
3D Structure
ModBase 3D Structure for O00459
Host
Synthetic
Species Reactivity
Human
Specificity
PIK3R2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Source
Synthetic
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PIK3R2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of PIK3R2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PIK3R2 sirna
siRNA to inhibit PIK3R2 expression using RNA interference
Applications Tested/Suitable for PIK3R2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for PIK3R2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
81,545 Da
NCBI Official Full Name
phosphatidylinositol 3-kinase regulatory subunit beta
NCBI Official Synonym Full Names
phosphoinositide-3-kinase, regulatory subunit 2 (beta)
NCBI Official Symbol
PIK3R2  [Similar Products]
NCBI Official Synonym Symbols
p85; MPPH; P85B; MPPH1; p85-BETA
  [Similar Products]
NCBI Protein Information
phosphatidylinositol 3-kinase regulatory subunit beta
UniProt Protein Name
Phosphatidylinositol 3-kinase regulatory subunit beta
UniProt Synonym Protein Names
Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; PI3-kinase subunit p85-beta; PtdIns-3-kinase regulatory subunit p85-beta
UniProt Gene Name
PIK3R2  [Similar Products]
UniProt Synonym Gene Names
PI3-kinase regulatory subunit beta; PI3K regulatory subunit beta; PtdIns-3-kinase regulatory subunit beta; PI3-kinase subunit p85-beta; PtdIns-3-kinase regulatory subunit p85-beta  [Similar Products]
UniProt Entry Name
P85B_HUMAN
NCBI Summary for PIK3R2
Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates phosphatidylinositol and similar compounds, creating second messengers important in growth signaling pathways. PI3K functions as a heterodimer of a regulatory and a catalytic subunit. The protein encoded by this gene is a regulatory component of PI3K. Two transcript variants, one protein coding and the other non-protein coding, have been found for this gene. [provided by RefSeq, Dec 2012]
UniProt Comments for PIK3R2
PIK3R2: regulatory subunit of phosphoinositide-3-kinase. Mediates binding to a subset of tyrosine-phosphorylated proteins through its SH2 domain. Acts as an adapter, mediating the association of the p110 catalytic unit of the alpha, beta and delta enzymes to the plasma membrane, where p110 phosphorylates inositol lipids. May play an additional role in the regulation of the actin cytoskeleton. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Its SH2 domains interacts with the YTHM motif of phosphorylated INSR in vitro. Defects in PIK3R1 are a cause of severe insulin resistance. Four alternatively spliced isoforms have been described.

Protein type: Kinase, lipid; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19q13.2-q13.4

Cellular Component: nucleus; cytosol; phosphoinositide 3-kinase complex

Molecular Function: protein binding; protein heterodimerization activity; 1-phosphatidylinositol-3-kinase activity; phosphoinositide 3-kinase regulator activity; receptor tyrosine kinase binding; protein phosphatase binding

Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide 3-kinase cascade; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; regulation of phosphoinositide 3-kinase activity; T cell receptor signaling pathway; protein transport; regulation of small GTPase mediated signal transduction; cellular response to insulin stimulus; phospholipid metabolic process; small GTPase mediated signal transduction; insulin receptor signaling pathway; phosphatidylinositol biosynthetic process; positive regulation of transcription factor import into nucleus; cell glucose homeostasis; innate immune response; regulation of autophagy; positive regulation of transcription from RNA polymerase II promoter; vascular endothelial growth factor receptor signaling pathway; blood coagulation; leukocyte migration

Disease: Megalencephaly-polymicrogyria-polydactyly-hydrocephalus Syndrome 1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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