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PIN4 elisa kit :: Mouse PIN4 ELISA Kit

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Catalog # MBS2515090
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

PIN4, ELISA Kit

Popular Item
Also Known As

Mouse PIN4 (Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting 4 Protein) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
133
OMIM
300252
3D Structure
ModBase 3D Structure for Q9Y237
Species Reactivity
Specificity
This kit recognizes natural and recombinant Mouse PIN4. No significant cross-reactivity or interference between Mouse PIN4 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
Min: 0.094ng/mL; Max: 10ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PIN4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPIN4purchase
MBS2515090 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PIN4, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PIN4. The ELISA analytical biochemical technique of the MBS2515090 kit is based on PIN4 antibody-PIN4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PIN4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PIN4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PIN4 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse PIN4 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PIN4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PIN4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PIN4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PIN4. You can calculate the concentration of PIN4 in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of PIN4 elisa kit
PIN4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2515090. Click to request current manual
NCBI/Uniprot data below describe general gene information for PIN4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
14,179 Da
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 isoform 2
NCBI Official Synonym Full Names
protein (peptidylprolyl cis/trans isomerase) NIMA-interacting, 4 (parvulin)
NCBI Official Symbol
NCBI Official Synonym Symbols
EPVH; PAR14; PAR17
  [Similar Products]
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase NIMA-interacting 4; PPIase PIN4; eukaryotic parvulin homolog; hEPVH; hPar14; hPar17; parvulin-14; parvulin-17; peptidyl-prolyl cis-trans isomerase Pin4; peptidyl-prolyl cis/trans isomerase EPVH; rotamase PIN4
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4
UniProt Synonym Protein Names
Parvulin-14; Par14; hPar14; Parvulin-17; Par17; hPar17; Peptidyl-prolyl cis-trans isomerase Pin4; PPIase Pin4; Peptidyl-prolyl cis/trans isomerase EPVH; hEPVH; Rotamase Pin4
UniProt Gene Name
UniProt Synonym Gene Names
Par14; hPar14; Par17; hPar17; PPIase Pin4; hEPVH  [Similar Products]
UniProt Entry Name
PIN4_HUMAN
NCBI Summary for PIN4
This gene encodes a member of the parvulin subfamily of the peptidyl-prolyl cis/trans isomerase protein family. The encoded protein catalyzes the isomerization of peptidylprolyl bonds, and may play a role in the cell cycle, chromatin remodeling, and/or ribosome biogenesis. The encoded protein may play an additional role in the mitochondria. [provided by RefSeq, Dec 2009]
UniProt Comments for PIN4
PIN4: Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA. Belongs to the PpiC/parvulin rotamase family. PIN4 subfamily. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: EC 5.2.1.8; Isomerase; Nucleolus; Mitochondrial

Chromosomal Location of Human Ortholog: Xq13

Cellular Component: mitochondrial matrix; cytoplasm; nucleolus; spindle; nucleus

Molecular Function: peptidyl-prolyl cis-trans isomerase activity; DNA binding; double-stranded DNA binding; bent DNA binding

Biological Process: protein peptidyl-prolyl isomerization; rRNA processing
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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