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PK2 elisa kit :: Rat PK2 ELISA Kit

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Catalog # MBS2507927
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  48-Strip-Wells  /  $410 +1 FREE 8GB USB
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  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

PK2, ELISA Kit

Popular Item
Full Product Name

Rat PK2 (Prokineticin 2) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
607002
3D Structure
ModBase 3D Structure for Q9HC23
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat PK2. No significant cross-reactivity or interference between Rat PK2 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rat PK2 and all the analogues, therefore, cross reaction may still exist.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.313-20ng/mL
Sensitivity
Min: 0.188ng/mL; Max: 20ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PK2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for PK2purchase
MBS2507927 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PK2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PK2. The ELISA analytical biochemical technique of the MBS2507927 kit is based on PK2 antibody-PK2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PK2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PK2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PK2 elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PK2 in Rat serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PK2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat PK2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PK2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat PK2. You can calculate the concentration of Rat PK2 in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of PK2 elisa kit
PK2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2507927. Click to request current manual
NCBI/Uniprot data below describe general gene information for PK2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
11,659 Da
NCBI Official Full Name
prokineticin-2 isoform a
NCBI Official Synonym Full Names
prokineticin 2
NCBI Official Symbol
PROK2  [Similar Products]
NCBI Official Synonym Symbols
BV8; HH4; PK2; KAL4; MIT1
  [Similar Products]
NCBI Protein Information
prokineticin-2; protein Bv8 homolog
UniProt Protein Name
Prokineticin-2
UniProt Synonym Protein Names
Protein Bv8 homolog
UniProt Gene Name
PROK2  [Similar Products]
UniProt Synonym Gene Names
BV8; PK2  [Similar Products]
UniProt Entry Name
PROK2_HUMAN
NCBI Summary for PK2
This gene encodes a protein expressed in the suprachiasmatic nucleus (SCN) circadian clock that may function as the output component of the circadian clock. The secreted form of the encoded protein may also serve as a chemoattractant for neuronal precursor cells in the olfactory bulb. Proteins from other vertebrates which are similar to this gene product were isolated based on homology to snake venom and secretions from frog skin, and have been shown to have diverse functions. Mutations in this gene are associated with Kallmann syndrome 4. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for PK2
PROK2: May function as an output molecule from the suprachiasmatic nucleus (SCN) that transmits behavioral circadian rhythm. May also function locally within the SCN to synchronize output. Potently contracts gastrointestinal (GI) smooth muscle. Defects in PROK2 are the cause of Kallmann syndrome type 4 (KAL4); also known as hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin- releasing hormone-synthesizing neurons. KAL4 patients have variable degrees of olfactory and reproductive dysfunction, but do not show any of the occasional clinical anomalies reported in Kallmann syndrome such as renal agenesis, cleft lip/palate, selective tooth agenesis, and bimanual synkinesis. Belongs to the AVIT (prokineticin) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3p13

Cellular Component: extracellular region

Molecular Function: G-protein-coupled receptor binding

Biological Process: circadian rhythm; activation of MAPK activity; chemotaxis; sensory perception of pain; G-protein coupled receptor protein signaling pathway; cell proliferation; elevation of cytosolic calcium ion concentration; neuropeptide signaling pathway; spermatogenesis; positive regulation of smooth muscle contraction; angiogenesis; inflammatory response; negative regulation of apoptosis

Disease: Hypogonadotropic Hypogonadism 4 With Or Without Anosmia
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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