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PKCb1 rtu elisa kit :: Rat Protein Kinase C Beta 1 (PKCb1) RTU ELISA Kit

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Catalog # MBS4501867
Unit / Price
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  48-Strip-Wells  /  $425 +1 FREE 8GB USB
  96-Strip-Wells  /  $570 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,600 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,050 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Protein Kinase C Beta 1 (PKCb1), RTU ELISA Kit

Full Product Name

Rat Protein Kinase C Beta 1 (PKCb1) ELISA Kit

Product Synonym Names
PKC-B1; PRKCB; PRKCB1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for P68403
Species Reactivity
Assay Type
Sandwich
Samples
Tissue Homogenates, Cell Lysates or Other Biological Fluids.
Detection Range
0.156-10ng/mL
Sensitivity
0.057ng/mL
RTU ELISA Kit's Advantage
The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our RTU ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PKCb1 rtu elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PKCb1 rtu elisa kit
Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PECAM1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PECAM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PECAM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PECAM1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of PKCb1 rtu elisa kit
PKCb1 rtu elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS4501867. Click to request current manual
NCBI/Uniprot data below describe general gene information for PKCb1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
76,894 Da
NCBI Official Full Name
protein kinase C beta type isoform 2
NCBI Official Synonym Full Names
protein kinase C, beta
NCBI Official Symbol
Prkcb  [Similar Products]
NCBI Official Synonym Symbols
Pkcb; Prkcb1
  [Similar Products]
NCBI Protein Information
protein kinase C beta type
UniProt Protein Name
Protein kinase C beta type
UniProt Gene Name
Prkcb  [Similar Products]
UniProt Synonym Gene Names
Pkcb; Prkcb1; PKC-B; PKC-beta  [Similar Products]
NCBI Summary for PKCb1
plays a role in hexosamine biosynthesis pathway induced transcriptional regulation [RGD, Feb 2006]
UniProt Comments for PKCb1
Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. May participate in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis (). Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with PKCb1 rtu elisa kit
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